Microfluidics platform for measurement of volume changes in immobilized intestinal enteroids

Byung Ju Jin, Sailaja Battula, Nick Zachos, Olga Kovbasnjuk, Jennifer Fawlke-Abel, Julie In, Mark Donowitz, Alan S. Verkman

Research output: Contribution to journalArticle

Abstract

Intestinal enteroids are ex vivo primary cultured single-layer epithelial cell spheroids of average diameter ~150 μm with luminal surface facing inward. Measurement of enteroid swelling in response to secretagogues has been applied to genetic testing in cystic fibrosis and evaluation of drug candidates for cystic fibrosis and secretory diarrheas. The current measurement method involves manual addition of drugs and solutions to enteroids embedded in a Matrigel matrix and estimation of volume changes from confocal images of fluorescently stained enteroids. We developed a microfluidics platform for efficient trapping and immobilization of enteroids for quantitative measurement of volume changes. Multiple enteroids are trapped in a "pinball machine-like" array of polydimethylsiloxane posts for measurement of volume changes in unlabeled enteroids by imaging of an extracellular, high-molecular weight fluorescent dye. Measurement accuracy was validated using slowly expanding air bubbles. The method was applied to measure swelling of mouse jejunal enteroids in response to an osmotic challenge and cholera toxin-induced chloride secretion. The microfluidics platform allows for parallel measurement of volume changes on multiple enteroids during continuous superfusion, without an immobilizing matrix, and for quantitative volume determination without chemical labeling or assumptions about enteroid shape changes during swelling.

Original languageEnglish (US)
Article number024106
JournalBiomicrofluidics
Volume8
Issue number2
DOIs
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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