Microfluidic two-dimensional IEF-CZE for proteomics

S. Yang, D. L. DeVoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Separations of complex proteomes are required to fractionate components before downstream identification and quantification of individual protein species. Twodimensional gels based on a combination of isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) have been a core technology for real-word detection of thousands of proteins, and microfluidic systems which mimic the functionality of 2-D gels in a miniature high-throughput format are a promising technology. Here we describe a new approach based on the combination of IEF with parallel capillary zone electrophoresis (CZE) in a microchip format, enabling improved reproducibility and higher throughput separations by eliminating the use of sieving gel in the second separation dimension. Although the system sacrifices some degree of resolving power through the use of non-orthogonal separation modes, overall peak capacities on the order of 2,500 are realized in the 2-D microfluidic chips.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages1578-1581
Number of pages4
DOIs
StatePublished - Dec 11 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Keywords

  • Capillary zone electrophoresis
  • Isoelectric focusing
  • Multiplexing
  • Proteomics
  • Separations

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Microfluidic two-dimensional IEF-CZE for proteomics'. Together they form a unique fingerprint.

  • Cite this

    Yang, S., & DeVoe, D. L. (2009). Microfluidic two-dimensional IEF-CZE for proteomics. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1578-1581). [5285789] (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285789