Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids

Amy Y. Hsiao, Yu suke Torisawa, Yi Chung Tung, Sudha Sud, Russell S. Taichman, Kenneth Pienta, Shuichi Takayama

Research output: Contribution to journalArticle

Abstract

The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.

Original languageEnglish (US)
Pages (from-to)3020-3027
Number of pages8
JournalBiomaterials
Volume30
Issue number16
DOIs
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Microfluidics
Coculture Techniques
Cell culture
Prostatic Neoplasms
Cells
Bone
Neoplasms
Osteoblasts
Endothelial cells
Tumors
Bone and Bones
Tumor Microenvironment
Engineers
Growth
Endothelial Cells
Cell Line

Keywords

  • 3D co-culture
  • Cancer stem cell
  • Microfluidics
  • Prostate cancer
  • Spheroid

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Hsiao, A. Y., Torisawa, Y. S., Tung, Y. C., Sud, S., Taichman, R. S., Pienta, K., & Takayama, S. (2009). Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. Biomaterials, 30(16), 3020-3027. https://doi.org/10.1016/j.biomaterials.2009.02.047

Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. / Hsiao, Amy Y.; Torisawa, Yu suke; Tung, Yi Chung; Sud, Sudha; Taichman, Russell S.; Pienta, Kenneth; Takayama, Shuichi.

In: Biomaterials, Vol. 30, No. 16, 06.2009, p. 3020-3027.

Research output: Contribution to journalArticle

Hsiao, AY, Torisawa, YS, Tung, YC, Sud, S, Taichman, RS, Pienta, K & Takayama, S 2009, 'Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids', Biomaterials, vol. 30, no. 16, pp. 3020-3027. https://doi.org/10.1016/j.biomaterials.2009.02.047
Hsiao, Amy Y. ; Torisawa, Yu suke ; Tung, Yi Chung ; Sud, Sudha ; Taichman, Russell S. ; Pienta, Kenneth ; Takayama, Shuichi. / Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. In: Biomaterials. 2009 ; Vol. 30, No. 16. pp. 3020-3027.
@article{8cd71060d17443e18332ba648b135f62,
title = "Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids",
abstract = "The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.",
keywords = "3D co-culture, Cancer stem cell, Microfluidics, Prostate cancer, Spheroid",
author = "Hsiao, {Amy Y.} and Torisawa, {Yu suke} and Tung, {Yi Chung} and Sudha Sud and Taichman, {Russell S.} and Kenneth Pienta and Shuichi Takayama",
year = "2009",
month = "6",
doi = "10.1016/j.biomaterials.2009.02.047",
language = "English (US)",
volume = "30",
pages = "3020--3027",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "16",

}

TY - JOUR

T1 - Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids

AU - Hsiao, Amy Y.

AU - Torisawa, Yu suke

AU - Tung, Yi Chung

AU - Sud, Sudha

AU - Taichman, Russell S.

AU - Pienta, Kenneth

AU - Takayama, Shuichi

PY - 2009/6

Y1 - 2009/6

N2 - The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.

AB - The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.

KW - 3D co-culture

KW - Cancer stem cell

KW - Microfluidics

KW - Prostate cancer

KW - Spheroid

UR - http://www.scopus.com/inward/record.url?scp=63649097667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63649097667&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2009.02.047

DO - 10.1016/j.biomaterials.2009.02.047

M3 - Article

VL - 30

SP - 3020

EP - 3027

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 16

ER -