Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011

Mary Younan; Mee Kian Poh; Emad Elassal; Todd Davis; Pierre Rivailler; Amanda L. Balish; Natosha Simpson; Joyce Jones; Varough Deyde; Rosette Loughlin; Ije Perry; Larisa Gubareva; Maha A. ElBadry; Shaun Truelove; Anne M. Gaynor; Emad Mohareb; Magdy Amin; Claire Cornelius; Guillermo Pimentel; Kenneth Earhart; Amel Naguib; Ahmed S. Abdelghani; Samir Refaey; Alexander I. Klimov; Ruben O. Donis; Amr Kandeel

doi:10.3201/eid1901.121080

Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011

Mary Younan, Mee Kian Poh, Emad Elassal, Todd Davis, Pierre Rivailler, Amanda L. Balish, Natosha Simpson, Joyce Jones, Varough Deyde, Rosette Loughlin, Ije Perry, Larisa Gubareva, Maha A. ElBadry, Shaun Truelove, Anne M. Gaynor, Emad Mohareb, Magdy Amin, Claire Cornelius, Guillermo Pimentel, Kenneth EarhartAmel Naguib, Ahmed S. Abdelghani, Samir Refaey, Alexander I. Klimov, Ruben O. Donis, Amr Kandeel

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We analyzed highly pathogenic avian influenza A(H5N1) viruses isolated from humans infected in Egypt during 2007-2011. All analyzed viruses evolved from the lineage of subtype H5N1 viruses introduced into Egypt in 2006; we found minimal evidence of reassortment and no exotic introductions. The hemagglutinin genes of the viruses from 2011 formed a monophyletic group within clade 2.2.1 that also included human viruses from 2009 and 2010 and contemporary viruses from poultry; this finding is consistent with zoonotic transmission. Although molecular markers suggestive of decreased susceptibility to antiviral drugs were detected sporadically in the neuraminidase and matrix 2 proteins, functional neuraminidase inhibition assays did not identify resistant viruses. No other mutations suggesting a change in the threat to public health were detected in the viral proteomes. However, a comparison of representative subtype H5N1 viruses from 2011 with older subtype H5N1 viruses from Egypt revealed substantial antigenic drift.

Original language	English (US)
Pages (from-to)	43-50
Number of pages	8
Journal	Emerging infectious diseases
Volume	19
Issue number	1
DOIs	https://doi.org/10.3201/eid1901.121080
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

Access to Document

10.3201/eid1901.121080

Cite this

Younan, M., Poh, M. K., Elassal, E., Davis, T., Rivailler, P., Balish, A. L., Simpson, N., Jones, J., Deyde, V., Loughlin, R., Perry, I., Gubareva, L., ElBadry, M. A., Truelove, S., Gaynor, A. M., Mohareb, E., Amin, M., Cornelius, C., Pimentel, G., ... Kandeel, A. (2013). Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011. Emerging infectious diseases, 19(1), 43-50. https://doi.org/10.3201/eid1901.121080

Younan, M, Poh, MK, Elassal, E, Davis, T, Rivailler, P, Balish, AL, Simpson, N, Jones, J, Deyde, V, Loughlin, R, Perry, I, Gubareva, L, ElBadry, MA, Truelove, S, Gaynor, AM, Mohareb, E, Amin, M, Cornelius, C, Pimentel, G, Earhart, K, Naguib, A, Abdelghani, AS, Refaey, S, Klimov, AI, Donis, RO & Kandeel, A 2013, 'Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011', Emerging infectious diseases, vol. 19, no. 1, pp. 43-50. https://doi.org/10.3201/eid1901.121080

@article{be04c917e2154ebdb4b83803ac6fa713,

title = "Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011",

abstract = "We analyzed highly pathogenic avian influenza A(H5N1) viruses isolated from humans infected in Egypt during 2007-2011. All analyzed viruses evolved from the lineage of subtype H5N1 viruses introduced into Egypt in 2006; we found minimal evidence of reassortment and no exotic introductions. The hemagglutinin genes of the viruses from 2011 formed a monophyletic group within clade 2.2.1 that also included human viruses from 2009 and 2010 and contemporary viruses from poultry; this finding is consistent with zoonotic transmission. Although molecular markers suggestive of decreased susceptibility to antiviral drugs were detected sporadically in the neuraminidase and matrix 2 proteins, functional neuraminidase inhibition assays did not identify resistant viruses. No other mutations suggesting a change in the threat to public health were detected in the viral proteomes. However, a comparison of representative subtype H5N1 viruses from 2011 with older subtype H5N1 viruses from Egypt revealed substantial antigenic drift.",

author = "Mary Younan and Poh, {Mee Kian} and Emad Elassal and Todd Davis and Pierre Rivailler and Balish, {Amanda L.} and Natosha Simpson and Joyce Jones and Varough Deyde and Rosette Loughlin and Ije Perry and Larisa Gubareva and ElBadry, {Maha A.} and Shaun Truelove and Gaynor, {Anne M.} and Emad Mohareb and Magdy Amin and Claire Cornelius and Guillermo Pimentel and Kenneth Earhart and Amel Naguib and Abdelghani, {Ahmed S.} and Samir Refaey and Klimov, {Alexander I.} and Donis, {Ruben O.} and Amr Kandeel",

year = "2013",

month = jan,

doi = "10.3201/eid1901.121080",

language = "English (US)",

volume = "19",

pages = "43--50",

journal = "Emerging infectious diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention (CDC)",

number = "1",

}

TY - JOUR

T1 - Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011

AU - Younan, Mary

AU - Poh, Mee Kian

AU - Elassal, Emad

AU - Davis, Todd

AU - Rivailler, Pierre

AU - Balish, Amanda L.

AU - Simpson, Natosha

AU - Jones, Joyce

AU - Deyde, Varough

AU - Loughlin, Rosette

AU - Perry, Ije

AU - Gubareva, Larisa

AU - ElBadry, Maha A.

AU - Truelove, Shaun

AU - Gaynor, Anne M.

AU - Mohareb, Emad

AU - Amin, Magdy

AU - Cornelius, Claire

AU - Pimentel, Guillermo

AU - Earhart, Kenneth

AU - Naguib, Amel

AU - Abdelghani, Ahmed S.

AU - Refaey, Samir

AU - Klimov, Alexander I.

AU - Donis, Ruben O.

AU - Kandeel, Amr

PY - 2013/1

Y1 - 2013/1

N2 - We analyzed highly pathogenic avian influenza A(H5N1) viruses isolated from humans infected in Egypt during 2007-2011. All analyzed viruses evolved from the lineage of subtype H5N1 viruses introduced into Egypt in 2006; we found minimal evidence of reassortment and no exotic introductions. The hemagglutinin genes of the viruses from 2011 formed a monophyletic group within clade 2.2.1 that also included human viruses from 2009 and 2010 and contemporary viruses from poultry; this finding is consistent with zoonotic transmission. Although molecular markers suggestive of decreased susceptibility to antiviral drugs were detected sporadically in the neuraminidase and matrix 2 proteins, functional neuraminidase inhibition assays did not identify resistant viruses. No other mutations suggesting a change in the threat to public health were detected in the viral proteomes. However, a comparison of representative subtype H5N1 viruses from 2011 with older subtype H5N1 viruses from Egypt revealed substantial antigenic drift.

AB - We analyzed highly pathogenic avian influenza A(H5N1) viruses isolated from humans infected in Egypt during 2007-2011. All analyzed viruses evolved from the lineage of subtype H5N1 viruses introduced into Egypt in 2006; we found minimal evidence of reassortment and no exotic introductions. The hemagglutinin genes of the viruses from 2011 formed a monophyletic group within clade 2.2.1 that also included human viruses from 2009 and 2010 and contemporary viruses from poultry; this finding is consistent with zoonotic transmission. Although molecular markers suggestive of decreased susceptibility to antiviral drugs were detected sporadically in the neuraminidase and matrix 2 proteins, functional neuraminidase inhibition assays did not identify resistant viruses. No other mutations suggesting a change in the threat to public health were detected in the viral proteomes. However, a comparison of representative subtype H5N1 viruses from 2011 with older subtype H5N1 viruses from Egypt revealed substantial antigenic drift.

UR - http://www.scopus.com/inward/record.url?scp=84871637350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871637350&partnerID=8YFLogxK

U2 - 10.3201/eid1901.121080

DO - 10.3201/eid1901.121080

M3 - Article

C2 - 23260983

AN - SCOPUS:84871637350

SN - 1080-6040

VL - 19

SP - 43

EP - 50

JO - Emerging infectious diseases

JF - Emerging infectious diseases

IS - 1

ER -

Microevolution of highly pathogenic avian influenza A(H5N1) viruses isolated from humans, Egypt, 2007-2011

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this