Microdissection Based Cloning of a Translocation Breakpoint in a Human Malignant Melanoma

Ji Zhang, Ping Cui, Arthur A. Glatfelter, Leda M. Cummings, Paul S. Meltzer, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosome translocations in human malignancies have identified the genomic location of several important growth-regulatory sequences (e.g., cellular oncogenes and suppressor genes). Melanomas are characterized by recurring chromosome alterations, including deletion or translocations of the long arm of chromosome 6 (6q). This report details our efforts to clone the t(1;6)(q21;ql4) breakpoint in a malignant melanoma to further our understanding of the biology of these tumors. The strategy utilized combined microdissection of the translocation chromosome, development and characterization of a DNA microclone library, isolation of cosmids and YACs from the breakpoint region, ordering of clones by two-color metaphase/interphase fluorescence in situ hybridization, and finally, identification of a YAC spanning the translocation breakpoint. By analogy to other tumor systems, molecular examination of the chromosome 6 breakpoint may provide insight into the pathobiology of this important neoplasm.

Original languageEnglish (US)
Pages (from-to)4640-4645
Number of pages6
JournalCancer Research
Volume55
Issue number20
StatePublished - Oct 15 1995

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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