Microdissection based cloning of a translocation breakpoint in a human malignant melanoma

J. Zhang, P. Cui, A. A. Glatfelter, L. M. Cummings, P. S. Meltzer, J. M. Trent

Research output: Contribution to journalArticle

Abstract

Chromosome translocations in human malignancies have identified the genomic location of several important growth-regulatory sequences (e.g., cellular oncogenes and suppressor genes). Melanomas are characterized by recurring chromosome alterations, including deletion or translocations of the long arm of chromosome 6 (6q). This report details our efforts to clone the t(1;6)(q21:q14) breakpoint in a malignant melanoma to further our understanding of the biology of these tumors. The strategy utilized combined microdissection of the translocation chromosome, development and characterization of a DNA microclone library, isolation of cosmids and YACs from the breakpoint region, ordering of clones by two-color metaphase/interphase fluorescence in situ hybridization, and finally, identification of a YAC spanning the translocation breakpoint. By analogy to other tumor systems, molecular examination of the chromosome 6 breakpoint may provide insight into the pathobiology of this important neoplasm.

Original languageEnglish (US)
Pages (from-to)4640-4645
Number of pages6
JournalCancer Research
Volume55
Issue number20
StatePublished - 1995
Externally publishedYes

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Microdissection
Organism Cloning
Melanoma
Chromosomes, Human, Pair 6
Chromosomes
Neoplasms
Clone Cells
Chromosome Breakpoints
Suppressor Genes
Cosmids
Interphase
Metaphase
Fluorescence In Situ Hybridization
Gene Library
Oncogenes
Color
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zhang, J., Cui, P., Glatfelter, A. A., Cummings, L. M., Meltzer, P. S., & Trent, J. M. (1995). Microdissection based cloning of a translocation breakpoint in a human malignant melanoma. Cancer Research, 55(20), 4640-4645.

Microdissection based cloning of a translocation breakpoint in a human malignant melanoma. / Zhang, J.; Cui, P.; Glatfelter, A. A.; Cummings, L. M.; Meltzer, P. S.; Trent, J. M.

In: Cancer Research, Vol. 55, No. 20, 1995, p. 4640-4645.

Research output: Contribution to journalArticle

Zhang, J, Cui, P, Glatfelter, AA, Cummings, LM, Meltzer, PS & Trent, JM 1995, 'Microdissection based cloning of a translocation breakpoint in a human malignant melanoma', Cancer Research, vol. 55, no. 20, pp. 4640-4645.
Zhang J, Cui P, Glatfelter AA, Cummings LM, Meltzer PS, Trent JM. Microdissection based cloning of a translocation breakpoint in a human malignant melanoma. Cancer Research. 1995;55(20):4640-4645.
Zhang, J. ; Cui, P. ; Glatfelter, A. A. ; Cummings, L. M. ; Meltzer, P. S. ; Trent, J. M. / Microdissection based cloning of a translocation breakpoint in a human malignant melanoma. In: Cancer Research. 1995 ; Vol. 55, No. 20. pp. 4640-4645.
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