Microarray-based phospho-proteomic profiling of complex biological systems

C. Rory Goodwin, Crystal L. Woodard, Xin Zhou, Jianbo Pan, Alessandro Olivi, Shuli Xia, Chetan Bettegowda, Daniel M. Sciubba, Jonathan Pevsner, Heng Zhu, John Laterra

Research output: Contribution to journalArticlepeer-review

Abstract

Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.

Original languageEnglish (US)
Pages (from-to)124-129
Number of pages6
JournalTranslational Oncology
Volume9
Issue number2
DOIs
StatePublished - Apr 1 2016

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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