Abstract
Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 124-129 |
| Number of pages | 6 |
| Journal | Translational Oncology |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1 2016 |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Microarray-based phospho-proteomic profiling of complex biological systems. / Goodwin, C. Rory; Woodard, Crystal L.; Zhou, Xin; Pan, Jianbo; Olivi, Alessandro; Xia, Shuli; Bettegowda, Chetan; Sciubba, Daniel M.; Pevsner, Jonathan; Zhu, Heng; Laterra, John.
In: Translational Oncology, Vol. 9, No. 2, 01.04.2016, p. 124-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microarray-based phospho-proteomic profiling of complex biological systems
AU - Goodwin,C. Rory
AU - Woodard,Crystal L.
AU - Zhou,Xin
AU - Pan,Jianbo
AU - Olivi,Alessandro
AU - Xia,Shuli
AU - Bettegowda,Chetan
AU - Sciubba,Daniel M.
AU - Pevsner,Jonathan
AU - Zhu,Heng
AU - Laterra,John
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.
AB - Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.
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UR - http://www.scopus.com/inward/citedby.url?scp=84963616109&partnerID=8YFLogxK
U2 - 10.1016/j.tranon.2016.02.001
DO - 10.1016/j.tranon.2016.02.001
M3 - Article
VL - 9
SP - 124
EP - 129
JO - Translational Oncology
T2 - Translational Oncology
JF - Translational Oncology
SN - 1936-5233
IS - 2
ER -