TY - JOUR
T1 - Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells
AU - Weigel, Andrea L.
AU - Handa, James T.
AU - Hjelmeland, Leonard M.
N1 - Funding Information:
This work was supported by: NIH/EY06473 (L.M.H.), NIH/EY14005 (J.T.H.), Research to Prevent Blindness Manpower Award (J.T.H.), and an unrestricted grant from Research to Prevent Blindness (Department of Ophthalmology, University of California, Davis). Supplementary data not published here will be available at the authors’ website, http://www.mcb.ucdavis.edu/faculty-labs/hjelmeland/ . We would like to thank Dr. Sharon Boylan for helpful discussions and critical reading of the manuscript.
PY - 2002/11/15
Y1 - 2002/11/15
N2 - Oxidative stress plays a key role in aging diseases of the posterior pole of the eye such as age-related macular degeneration. The oxidative stress response of in vitro RPE cells has been studied for a small number of genes. However, a comprehensive transcriptional response has yet to be elucidated. The purpose of this study was to determine if the transcription of a common set of genes is altered by exposure of ARPE-19 cells to three major generators of oxidative stress, hydrogen peroxide (H2O2), 4-hydroxynonenal (HNE), and tert-butylhydroperoxide (tBH). As expected, a common response was observed that included 35 genes differentially regulated by all three treatments. Of these, only one gene was upregulated, and only by one oxidant, while all other responses were downregulation. The majority of these genes fell into five functional categories: apoptosis, cell cycle regulation, cell-cell communication, signal transduction, and transcriptional regulation. Additionally, a large number of genes were differentially regulated by one oxidant only, including the majority of the conventional oxidative stress response genes present on the Clontech Human 1.2 microarray. This study raises questions regarding the generality of results that involve the use of a single oxidant and a single cell culture condition.
AB - Oxidative stress plays a key role in aging diseases of the posterior pole of the eye such as age-related macular degeneration. The oxidative stress response of in vitro RPE cells has been studied for a small number of genes. However, a comprehensive transcriptional response has yet to be elucidated. The purpose of this study was to determine if the transcription of a common set of genes is altered by exposure of ARPE-19 cells to three major generators of oxidative stress, hydrogen peroxide (H2O2), 4-hydroxynonenal (HNE), and tert-butylhydroperoxide (tBH). As expected, a common response was observed that included 35 genes differentially regulated by all three treatments. Of these, only one gene was upregulated, and only by one oxidant, while all other responses were downregulation. The majority of these genes fell into five functional categories: apoptosis, cell cycle regulation, cell-cell communication, signal transduction, and transcriptional regulation. Additionally, a large number of genes were differentially regulated by one oxidant only, including the majority of the conventional oxidative stress response genes present on the Clontech Human 1.2 microarray. This study raises questions regarding the generality of results that involve the use of a single oxidant and a single cell culture condition.
KW - Free radicals
KW - HNE
KW - HO
KW - Oxidative stress
KW - RPE cells
KW - cDNA microarray
KW - tBH
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U2 - 10.1016/S0891-5849(02)01082-1
DO - 10.1016/S0891-5849(02)01082-1
M3 - Article
C2 - 12419474
AN - SCOPUS:0037111462
SN - 0891-5849
VL - 33
SP - 1419
EP - 1432
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 10
ER -