Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells

Andrea L. Weigel, James Handa, Leonard M. Hjelmeland

Research output: Contribution to journalArticle

Abstract

Oxidative stress plays a key role in aging diseases of the posterior pole of the eye such as age-related macular degeneration. The oxidative stress response of in vitro RPE cells has been studied for a small number of genes. However, a comprehensive transcriptional response has yet to be elucidated. The purpose of this study was to determine if the transcription of a common set of genes is altered by exposure of ARPE-19 cells to three major generators of oxidative stress, hydrogen peroxide (H2O2), 4-hydroxynonenal (HNE), and tert-butylhydroperoxide (tBH). As expected, a common response was observed that included 35 genes differentially regulated by all three treatments. Of these, only one gene was upregulated, and only by one oxidant, while all other responses were downregulation. The majority of these genes fell into five functional categories: apoptosis, cell cycle regulation, cell-cell communication, signal transduction, and transcriptional regulation. Additionally, a large number of genes were differentially regulated by one oxidant only, including the majority of the conventional oxidative stress response genes present on the Clontech Human 1.2 microarray. This study raises questions regarding the generality of results that involve the use of a single oxidant and a single cell culture condition.

Original languageEnglish (US)
Pages (from-to)1419-1432
Number of pages14
JournalFree Radical Biology and Medicine
Volume33
Issue number10
DOIs
StatePublished - Nov 15 2002

Fingerprint

tert-Butylhydroperoxide
Microarray Analysis
Microarrays
Genes
Oxidative stress
Oxidative Stress
Oxidants
Signal transduction
Macular Degeneration
Transcription
Cell culture
Cell Communication
Hydrogen Peroxide
Poles
Signal Transduction
Cell Cycle
Down-Regulation
Cell Culture Techniques
Aging of materials
Cells

Keywords

  • cDNA microarray
  • Free radicals
  • HO
  • HNE
  • Oxidative stress
  • RPE cells
  • tBH

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells. / Weigel, Andrea L.; Handa, James; Hjelmeland, Leonard M.

In: Free Radical Biology and Medicine, Vol. 33, No. 10, 15.11.2002, p. 1419-1432.

Research output: Contribution to journalArticle

Weigel, Andrea L. ; Handa, James ; Hjelmeland, Leonard M. / Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells. In: Free Radical Biology and Medicine. 2002 ; Vol. 33, No. 10. pp. 1419-1432.
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