TY - JOUR
T1 - Microanalysis of ozone depression of motor activity
AU - Tepper, Jeffrey L.
AU - Weiss, Bernard
AU - Cox, Christopher
N1 - Funding Information:
’ This work was supported by NIH Pharmacological Sciences Training Grant 5 T32 GM-07141, by a grant from NIEHS (ES-10247), and by Contract DE-ACOZ-76EV03490 with The U.S. Department of Energy at the University of Rochester Department of Radiation Biology and Biophysics and has been assigned Report No. UR-3490-2079. Parts of this work were presented at the 20th Annual Meeting of the Society of Toxicology, March 1-5, 1981, in San Diego, Calif. ’ Submitted in partial fulfillment of the requirements for the Ph.D., Department of Pharmacology and Toxicology, University of Rochester. 3 Department of Pharmacology and Toxicology. 4 To whom requests for reprints should be addressed. 5 Division of Toxicology and Environmental Health Sciences Center, Department of Radiation Biology and Biophysics. 6 Division of Biostatistics.
PY - 1982/6/30
Y1 - 1982/6/30
N2 - Ozone, the principal oxidant in photochemical smog, impairs athletic performance and induces complaints of fatigue and lethargy. It also reduces motor activity in rodents. A detailed analysis of this finding was attempted. Eight male Long-Evans rats were housed in cages attached to running wheels located within a 2-m3 exposure chamber. Each revolution of a wheel closed a switch with the time between switch closures recorded by an attached computer. The rats were exposed for 6-hr periods during the nocturnal phase of their light cycle to ozone concentrations of 0.12, 0.25, 0.50, and 1.0 ppm. Five days or more separated successive exposures. The 3 days preceding an exposure served as control observations. Ozone produced initial decrements in the number of revolutions and a progressively greater decrease with continued exposure. Statistically significant depression took place at 0.12 ppm. Analysis of the individual components of wheel running revealed differential susceptibility to ozone. An increase in the interval between bursts of running was primarily responsible for the decrease in the number of revolutions; this interval was also more sensitive to disruption than either time per revolution or burst length at low concentrations. After termination of exposure to low concentrations of ozone, animals showed increased running. At the higher concentrations, running remained suppressed below control values for several hours. A new multivariate graphical technique, the biplot, is presented as a way to simultaneously display the relationships among many complex variables.
AB - Ozone, the principal oxidant in photochemical smog, impairs athletic performance and induces complaints of fatigue and lethargy. It also reduces motor activity in rodents. A detailed analysis of this finding was attempted. Eight male Long-Evans rats were housed in cages attached to running wheels located within a 2-m3 exposure chamber. Each revolution of a wheel closed a switch with the time between switch closures recorded by an attached computer. The rats were exposed for 6-hr periods during the nocturnal phase of their light cycle to ozone concentrations of 0.12, 0.25, 0.50, and 1.0 ppm. Five days or more separated successive exposures. The 3 days preceding an exposure served as control observations. Ozone produced initial decrements in the number of revolutions and a progressively greater decrease with continued exposure. Statistically significant depression took place at 0.12 ppm. Analysis of the individual components of wheel running revealed differential susceptibility to ozone. An increase in the interval between bursts of running was primarily responsible for the decrease in the number of revolutions; this interval was also more sensitive to disruption than either time per revolution or burst length at low concentrations. After termination of exposure to low concentrations of ozone, animals showed increased running. At the higher concentrations, running remained suppressed below control values for several hours. A new multivariate graphical technique, the biplot, is presented as a way to simultaneously display the relationships among many complex variables.
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U2 - 10.1016/0041-008X(82)90226-5
DO - 10.1016/0041-008X(82)90226-5
M3 - Article
C2 - 7123558
AN - SCOPUS:0019955981
SN - 0041-008X
VL - 64
SP - 317
EP - 326
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 2
ER -