Microallelotyping defines the sequence and tempo of allelic losses at tumour suppressor gene loci during colorectal cancer progression

C. R. Boland, J. Sato, H. D. Appelman, R. S. Bresalier, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

Microallelotyping of many regions from individual colorectal tumours was used to determine the sequence and tempo of allelic loss on 5q, 17p and 18q during neoplastic progression. No allelic losses were found in normal tissues surrounding colorectal neoplasms, but losses occurred abruptly on 5q at the transition from normal colonic epithelium to the benign adenoma, and on 17p at the transition from adenoma to carcinoma, indicating an essential role for these losses in tumour progression. Allelic losses were uniform throughout extensively microdissected benign adenomas and carcinomas. However, substantial allelic heterogeneity was found in high-grade dysplasia, the transition lesion between adenoma and carcinoma. Thus, allelic losses on 5q and 17p are associated with abrupt waves of clonal neoplastic expansion, and high-grade dysplasia is characterized by a high degree of allelic heterogeneity.

Original languageEnglish (US)
Pages (from-to)902-909
Number of pages8
JournalNature Medicine
Volume1
Issue number9
StatePublished - 1995

Fingerprint

Loss of Heterozygosity
Tumor Suppressor Genes
Adenoma
Tumors
Colorectal Neoplasms
Genes
Carcinoma
Epithelium
Tissue
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Microallelotyping defines the sequence and tempo of allelic losses at tumour suppressor gene loci during colorectal cancer progression. / Boland, C. R.; Sato, J.; Appelman, H. D.; Bresalier, R. S.; Feinberg, Andrew P.

In: Nature Medicine, Vol. 1, No. 9, 1995, p. 902-909.

Research output: Contribution to journalArticle

@article{bac4a6a519594fd78ed03a061b97e3fb,
title = "Microallelotyping defines the sequence and tempo of allelic losses at tumour suppressor gene loci during colorectal cancer progression",
abstract = "Microallelotyping of many regions from individual colorectal tumours was used to determine the sequence and tempo of allelic loss on 5q, 17p and 18q during neoplastic progression. No allelic losses were found in normal tissues surrounding colorectal neoplasms, but losses occurred abruptly on 5q at the transition from normal colonic epithelium to the benign adenoma, and on 17p at the transition from adenoma to carcinoma, indicating an essential role for these losses in tumour progression. Allelic losses were uniform throughout extensively microdissected benign adenomas and carcinomas. However, substantial allelic heterogeneity was found in high-grade dysplasia, the transition lesion between adenoma and carcinoma. Thus, allelic losses on 5q and 17p are associated with abrupt waves of clonal neoplastic expansion, and high-grade dysplasia is characterized by a high degree of allelic heterogeneity.",
author = "Boland, {C. R.} and J. Sato and Appelman, {H. D.} and Bresalier, {R. S.} and Feinberg, {Andrew P}",
year = "1995",
language = "English (US)",
volume = "1",
pages = "902--909",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Microallelotyping defines the sequence and tempo of allelic losses at tumour suppressor gene loci during colorectal cancer progression

AU - Boland, C. R.

AU - Sato, J.

AU - Appelman, H. D.

AU - Bresalier, R. S.

AU - Feinberg, Andrew P

PY - 1995

Y1 - 1995

N2 - Microallelotyping of many regions from individual colorectal tumours was used to determine the sequence and tempo of allelic loss on 5q, 17p and 18q during neoplastic progression. No allelic losses were found in normal tissues surrounding colorectal neoplasms, but losses occurred abruptly on 5q at the transition from normal colonic epithelium to the benign adenoma, and on 17p at the transition from adenoma to carcinoma, indicating an essential role for these losses in tumour progression. Allelic losses were uniform throughout extensively microdissected benign adenomas and carcinomas. However, substantial allelic heterogeneity was found in high-grade dysplasia, the transition lesion between adenoma and carcinoma. Thus, allelic losses on 5q and 17p are associated with abrupt waves of clonal neoplastic expansion, and high-grade dysplasia is characterized by a high degree of allelic heterogeneity.

AB - Microallelotyping of many regions from individual colorectal tumours was used to determine the sequence and tempo of allelic loss on 5q, 17p and 18q during neoplastic progression. No allelic losses were found in normal tissues surrounding colorectal neoplasms, but losses occurred abruptly on 5q at the transition from normal colonic epithelium to the benign adenoma, and on 17p at the transition from adenoma to carcinoma, indicating an essential role for these losses in tumour progression. Allelic losses were uniform throughout extensively microdissected benign adenomas and carcinomas. However, substantial allelic heterogeneity was found in high-grade dysplasia, the transition lesion between adenoma and carcinoma. Thus, allelic losses on 5q and 17p are associated with abrupt waves of clonal neoplastic expansion, and high-grade dysplasia is characterized by a high degree of allelic heterogeneity.

UR - http://www.scopus.com/inward/record.url?scp=0029075933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029075933&partnerID=8YFLogxK

M3 - Article

C2 - 7585215

AN - SCOPUS:0029075933

VL - 1

SP - 902

EP - 909

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 9

ER -