MICER targeting vectors for manipulating the mouse genome.

Chunhong Liu, Paul F. Szurek, Y. Eugene Yu

Research output: Contribution to journalArticle

Abstract

The mouse has become an important model for understanding human development, physiology and disease because of its genetic and biological similarity to humans. Desired mouse mutants with precise genetic alterations can now be generated through gene targeting in mouse embryonic stem cells. The rate-limiting factor in a gene-targeting experiment is the time needed for cloning to construct targeting vectors. The establishment of the Mutagenic Insertion and Chromosome Engineering Resource has made available targeting vectors for the insertional mutagenesis of a large number of mouse genes as well as for chromosome engineering throughout the mouse genome. This unique resource has enriched the repertoire of the genetic reagents for targeted manipulation of the mouse genome.

Original languageEnglish (US)
Pages (from-to)245-256
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume693
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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