Mice deficient in AKAP13 (BRX) develop compulsive-like behavior and increased body weight

K. Maravet Baig, Szu Chi Su, Sunni L. Mumford, Emma Giuliani, Sinnie Sin Man Ng, Charles Armstrong, Margaret F. Keil, Kamaria Cayton Vaught, Nils Olsen, Elyse Pettiford, Irina Burd, James Segars

Research output: Contribution to journalArticle

Abstract

Objective: Hormonal contributions to the sex-dependent development of both obsessive-compulsive disorder (OCD) and obesity have been described, but the underlying mechanisms are incompletely understood. A-kinase anchoring protein 13 (AKAP13) significantly augments ligand-dependent activation of estrogen receptors alpha and beta. The hypothalamus and pituitary gland are implicated in the development and exacerbation of OCD and obesity and have strong AKAP13 expression. The AKAP13 localization pattern observed in these key brain regions together with its effects on sex steroid action suggest a potential role for AKAP13 in compulsive-like behaviors. Here we tested the role of AKAP13 in compulsive-like behavior and body weight using an Akap13 haploinsufficient murine model. Materials and methods: Targeted deletion of the Akap13 gene generated haploinsufficient (Akap13+/−) mice in a C57BL6/J genetic background. Established behavioral assays were conducted, video recorded, and scored blindly to assess compulsive-like behavior based on genotype and gender. Tests included: marble-burying, grooming, open- field and elevated plus-maze. Brain and body weights were also obtained. Mean levels of test outcomes were compared using multi-way ANOVA to test for genotype, sex, genotype*sex, and genotype*sex*age interaction effects with Bonferroni adjustment for multiple comparisons, to further explain any significant interactions. Results: The marble-burying and grooming assays revealed significant sex-dependent increases in perseverative, compulsive-like behaviors in female Akap13 haploinsufficient mice compared to female wild type (WT) mice by demonstrating increased marble-burying activity (p =.0025) and a trend towards increased grooming behavior (p =.06). Male Akap13 haploinsufficient mice exhibited no behavioral changes (p > 0.05). Elevated plus-maze and open-field test results showed no overt anxiety-like behavior in Akap13 haploinsufficient mice irrespective of sex (p > 0.05, both). No differences in brain weight were found in Akap13 haploinsufficient mice compared to WT mice (p > 0.05). However, female Akap13 haploinsufficient mice weighed more than female WT mice in the 4 to <7 months age range (p =.0051). Male Akap13 haploinsufficient mice showed no differences in weight compared to male WT mice (p = >0.05) at any age range examined. Conclusion: Akap13 haploinsufficiency led to sex-dependent, compulsive-like behavioral changes in a murine model. Interestingly, Akap13 haploinsufficiency also led to a sex-dependent increase in body weight. These results revealed a requirement for AKAP13 in murine behavior, particularly in female mice, and is the first report of AKAP13 involvement in murine behavior. Future studies may examine the involvement of AKAP13 in the pathophysiology of OCD in female humans and may contribute to a better understanding of the role of AKAP13 and sex hormones in the development and exacerbation of OCD.

Original languageEnglish (US)
Pages (from-to)72-79
Number of pages8
JournalBrain Research Bulletin
Volume140
DOIs
StatePublished - Jun 1 2018

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Compulsive Behavior
Protein Kinases
Body Weight
Obsessive-Compulsive Disorder
Grooming
Calcium Carbonate
Genotype
Haploinsufficiency
Brain
Obesity
Estrogen Receptor beta
Sexual Development
Estrogen Receptor alpha
Gene Deletion
Gonadal Steroid Hormones
Pituitary Gland
Hypothalamus
Analysis of Variance
Anxiety
Steroids

Keywords

  • AKAP13
  • Anxiety
  • BRX
  • Estrogen
  • Obesity
  • Obsessive-compulsive disorder

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mice deficient in AKAP13 (BRX) develop compulsive-like behavior and increased body weight. / Maravet Baig, K.; Su, Szu Chi; Mumford, Sunni L.; Giuliani, Emma; Ng, Sinnie Sin Man; Armstrong, Charles; Keil, Margaret F.; Vaught, Kamaria Cayton; Olsen, Nils; Pettiford, Elyse; Burd, Irina; Segars, James.

In: Brain Research Bulletin, Vol. 140, 01.06.2018, p. 72-79.

Research output: Contribution to journalArticle

Maravet Baig, K, Su, SC, Mumford, SL, Giuliani, E, Ng, SSM, Armstrong, C, Keil, MF, Vaught, KC, Olsen, N, Pettiford, E, Burd, I & Segars, J 2018, 'Mice deficient in AKAP13 (BRX) develop compulsive-like behavior and increased body weight', Brain Research Bulletin, vol. 140, pp. 72-79. https://doi.org/10.1016/j.brainresbull.2018.04.005
Maravet Baig, K. ; Su, Szu Chi ; Mumford, Sunni L. ; Giuliani, Emma ; Ng, Sinnie Sin Man ; Armstrong, Charles ; Keil, Margaret F. ; Vaught, Kamaria Cayton ; Olsen, Nils ; Pettiford, Elyse ; Burd, Irina ; Segars, James. / Mice deficient in AKAP13 (BRX) develop compulsive-like behavior and increased body weight. In: Brain Research Bulletin. 2018 ; Vol. 140. pp. 72-79.
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abstract = "Objective: Hormonal contributions to the sex-dependent development of both obsessive-compulsive disorder (OCD) and obesity have been described, but the underlying mechanisms are incompletely understood. A-kinase anchoring protein 13 (AKAP13) significantly augments ligand-dependent activation of estrogen receptors alpha and beta. The hypothalamus and pituitary gland are implicated in the development and exacerbation of OCD and obesity and have strong AKAP13 expression. The AKAP13 localization pattern observed in these key brain regions together with its effects on sex steroid action suggest a potential role for AKAP13 in compulsive-like behaviors. Here we tested the role of AKAP13 in compulsive-like behavior and body weight using an Akap13 haploinsufficient murine model. Materials and methods: Targeted deletion of the Akap13 gene generated haploinsufficient (Akap13+/−) mice in a C57BL6/J genetic background. Established behavioral assays were conducted, video recorded, and scored blindly to assess compulsive-like behavior based on genotype and gender. Tests included: marble-burying, grooming, open- field and elevated plus-maze. Brain and body weights were also obtained. Mean levels of test outcomes were compared using multi-way ANOVA to test for genotype, sex, genotype*sex, and genotype*sex*age interaction effects with Bonferroni adjustment for multiple comparisons, to further explain any significant interactions. Results: The marble-burying and grooming assays revealed significant sex-dependent increases in perseverative, compulsive-like behaviors in female Akap13 haploinsufficient mice compared to female wild type (WT) mice by demonstrating increased marble-burying activity (p =.0025) and a trend towards increased grooming behavior (p =.06). Male Akap13 haploinsufficient mice exhibited no behavioral changes (p > 0.05). Elevated plus-maze and open-field test results showed no overt anxiety-like behavior in Akap13 haploinsufficient mice irrespective of sex (p > 0.05, both). No differences in brain weight were found in Akap13 haploinsufficient mice compared to WT mice (p > 0.05). However, female Akap13 haploinsufficient mice weighed more than female WT mice in the 4 to <7 months age range (p =.0051). Male Akap13 haploinsufficient mice showed no differences in weight compared to male WT mice (p = >0.05) at any age range examined. Conclusion: Akap13 haploinsufficiency led to sex-dependent, compulsive-like behavioral changes in a murine model. Interestingly, Akap13 haploinsufficiency also led to a sex-dependent increase in body weight. These results revealed a requirement for AKAP13 in murine behavior, particularly in female mice, and is the first report of AKAP13 involvement in murine behavior. Future studies may examine the involvement of AKAP13 in the pathophysiology of OCD in female humans and may contribute to a better understanding of the role of AKAP13 and sex hormones in the development and exacerbation of OCD.",
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author = "{Maravet Baig}, K. and Su, {Szu Chi} and Mumford, {Sunni L.} and Emma Giuliani and Ng, {Sinnie Sin Man} and Charles Armstrong and Keil, {Margaret F.} and Vaught, {Kamaria Cayton} and Nils Olsen and Elyse Pettiford and Irina Burd and James Segars",
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TY - JOUR

T1 - Mice deficient in AKAP13 (BRX) develop compulsive-like behavior and increased body weight

AU - Maravet Baig, K.

AU - Su, Szu Chi

AU - Mumford, Sunni L.

AU - Giuliani, Emma

AU - Ng, Sinnie Sin Man

AU - Armstrong, Charles

AU - Keil, Margaret F.

AU - Vaught, Kamaria Cayton

AU - Olsen, Nils

AU - Pettiford, Elyse

AU - Burd, Irina

AU - Segars, James

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Objective: Hormonal contributions to the sex-dependent development of both obsessive-compulsive disorder (OCD) and obesity have been described, but the underlying mechanisms are incompletely understood. A-kinase anchoring protein 13 (AKAP13) significantly augments ligand-dependent activation of estrogen receptors alpha and beta. The hypothalamus and pituitary gland are implicated in the development and exacerbation of OCD and obesity and have strong AKAP13 expression. The AKAP13 localization pattern observed in these key brain regions together with its effects on sex steroid action suggest a potential role for AKAP13 in compulsive-like behaviors. Here we tested the role of AKAP13 in compulsive-like behavior and body weight using an Akap13 haploinsufficient murine model. Materials and methods: Targeted deletion of the Akap13 gene generated haploinsufficient (Akap13+/−) mice in a C57BL6/J genetic background. Established behavioral assays were conducted, video recorded, and scored blindly to assess compulsive-like behavior based on genotype and gender. Tests included: marble-burying, grooming, open- field and elevated plus-maze. Brain and body weights were also obtained. Mean levels of test outcomes were compared using multi-way ANOVA to test for genotype, sex, genotype*sex, and genotype*sex*age interaction effects with Bonferroni adjustment for multiple comparisons, to further explain any significant interactions. Results: The marble-burying and grooming assays revealed significant sex-dependent increases in perseverative, compulsive-like behaviors in female Akap13 haploinsufficient mice compared to female wild type (WT) mice by demonstrating increased marble-burying activity (p =.0025) and a trend towards increased grooming behavior (p =.06). Male Akap13 haploinsufficient mice exhibited no behavioral changes (p > 0.05). Elevated plus-maze and open-field test results showed no overt anxiety-like behavior in Akap13 haploinsufficient mice irrespective of sex (p > 0.05, both). No differences in brain weight were found in Akap13 haploinsufficient mice compared to WT mice (p > 0.05). However, female Akap13 haploinsufficient mice weighed more than female WT mice in the 4 to <7 months age range (p =.0051). Male Akap13 haploinsufficient mice showed no differences in weight compared to male WT mice (p = >0.05) at any age range examined. Conclusion: Akap13 haploinsufficiency led to sex-dependent, compulsive-like behavioral changes in a murine model. Interestingly, Akap13 haploinsufficiency also led to a sex-dependent increase in body weight. These results revealed a requirement for AKAP13 in murine behavior, particularly in female mice, and is the first report of AKAP13 involvement in murine behavior. Future studies may examine the involvement of AKAP13 in the pathophysiology of OCD in female humans and may contribute to a better understanding of the role of AKAP13 and sex hormones in the development and exacerbation of OCD.

AB - Objective: Hormonal contributions to the sex-dependent development of both obsessive-compulsive disorder (OCD) and obesity have been described, but the underlying mechanisms are incompletely understood. A-kinase anchoring protein 13 (AKAP13) significantly augments ligand-dependent activation of estrogen receptors alpha and beta. The hypothalamus and pituitary gland are implicated in the development and exacerbation of OCD and obesity and have strong AKAP13 expression. The AKAP13 localization pattern observed in these key brain regions together with its effects on sex steroid action suggest a potential role for AKAP13 in compulsive-like behaviors. Here we tested the role of AKAP13 in compulsive-like behavior and body weight using an Akap13 haploinsufficient murine model. Materials and methods: Targeted deletion of the Akap13 gene generated haploinsufficient (Akap13+/−) mice in a C57BL6/J genetic background. Established behavioral assays were conducted, video recorded, and scored blindly to assess compulsive-like behavior based on genotype and gender. Tests included: marble-burying, grooming, open- field and elevated plus-maze. Brain and body weights were also obtained. Mean levels of test outcomes were compared using multi-way ANOVA to test for genotype, sex, genotype*sex, and genotype*sex*age interaction effects with Bonferroni adjustment for multiple comparisons, to further explain any significant interactions. Results: The marble-burying and grooming assays revealed significant sex-dependent increases in perseverative, compulsive-like behaviors in female Akap13 haploinsufficient mice compared to female wild type (WT) mice by demonstrating increased marble-burying activity (p =.0025) and a trend towards increased grooming behavior (p =.06). Male Akap13 haploinsufficient mice exhibited no behavioral changes (p > 0.05). Elevated plus-maze and open-field test results showed no overt anxiety-like behavior in Akap13 haploinsufficient mice irrespective of sex (p > 0.05, both). No differences in brain weight were found in Akap13 haploinsufficient mice compared to WT mice (p > 0.05). However, female Akap13 haploinsufficient mice weighed more than female WT mice in the 4 to <7 months age range (p =.0051). Male Akap13 haploinsufficient mice showed no differences in weight compared to male WT mice (p = >0.05) at any age range examined. Conclusion: Akap13 haploinsufficiency led to sex-dependent, compulsive-like behavioral changes in a murine model. Interestingly, Akap13 haploinsufficiency also led to a sex-dependent increase in body weight. These results revealed a requirement for AKAP13 in murine behavior, particularly in female mice, and is the first report of AKAP13 involvement in murine behavior. Future studies may examine the involvement of AKAP13 in the pathophysiology of OCD in female humans and may contribute to a better understanding of the role of AKAP13 and sex hormones in the development and exacerbation of OCD.

KW - AKAP13

KW - Anxiety

KW - BRX

KW - Estrogen

KW - Obesity

KW - Obsessive-compulsive disorder

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