Mhc-ig induces memory t cell formation in vivo and inhibits tumour growth

Christian Schütz, Alessia Zoso, Shiwen Peng, Jonathon D. Bennett, Jonathan P. Schneck, Mathias Oelke

Research output: Contribution to journalArticlepeer-review

Abstract

Induction of a T cell mediated immune response is critical for the eradication of viral infections and tumours. Soluble peptide-loaded major histocompatibility complex-Ig (pep MHC-Ig) have been shown to bind their cognate ligands, T cell receptor, with high affinity, and are successfully used to visualize antigen-specific T cells. Furthermore, immobilizedpep MHC-Ig can activate and expand antigen-specific T cells in vitro and in vivo. In this study, we investigate the use ofpep MHC-Ig as a potential strategy to modulate antigen specific T cell immune responses in vivo.SIY Kb-Ig immunization, together with the pre-activation by an anti-CD40 monoclonal antibody, is able to stimulate a strong expansion of adoptively transferred 2C transgenic T cells and the formation of long term antigen-specific memory T cells. In addition, mechanistic studies show that thepep MHC-Ig molecules directly activate T cells in vivo without requiring uptake and reprocessing by antigen-presenting cells. Furthermore, B6 mice immunized withpep MHC-Ig molecules inhibit tumours growth in a B16-SIY melanoma prevention model. Thus, solublepep MHC-Ig molecules represent a powerful tool for active immunotherapy.

Original languageEnglish (US)
JournalImmunity, inflammation and disease
Volume2
Issue number3
DOIs
StatePublished - Nov 2014

Keywords

  • Adoptive T cell transfer
  • Cancer
  • In vivo vaccination
  • MHC-Ig
  • Memory T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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