MHC class I allele frequencies in pigtail macaques of diverse origin

Bridget F. Pratt, David H. O'Connor, Bernard A.P. Lafont, Joseph L. Mankowski, Caroline S. Fernandez, Retno Triastuti, Andrew G. Brooks, Stephen J. Kent, Miranda Z. Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Pigtail macaques (Macaca nemestrina) are an increasingly common primate model for the study of human AIDS. Major Histocompatibility complex (MHC) class I-restricted CD8+ T cell responses are a critical part of the adaptive immune response to HIV-1 in humans and simian immunodeficiency virus (SIV) in macaques; however, MHC class I alleles have not yet been comprehensively characterized in pigtail macaques. The frequencies of ten previously defined alleles (four Mane-A and six Mane-B) were investigated in detail in 109 pigtail macaques using reference strand-mediated conformational analysis (RSCA). The macaques were derived from three separate breeding colonies in the USA, Indonesia and Australia, and allele frequencies were analysed within and between these groups. Mane-A*10, an allele that restricts the immunodominant SIV Gag epitope KP9, was the most common allele, present in 32.1% of the animals overall, with similar frequencies across the three cohorts. Additionally, RSCA identified a new allele (Mane-A*17) common to three Indonesian pigtail macaques responding to the same Gag CD8+ T cell epitope. This broad characterization of common MHC class I alleles in more than 100 pigtail macaques further develops this animal model for the study of virus-specific CD8+ T cell responses.

Original languageEnglish (US)
Pages (from-to)995-1001
Number of pages7
JournalImmunogenetics
Volume58
Issue number12
DOIs
StatePublished - Dec 1 2006

Keywords

  • CD8 T cell
  • MHC class I
  • Pigtail macaques
  • Reference strand-mediated conformational analysis (RSCA)

ASJC Scopus subject areas

  • Immunology
  • Genetics

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