MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion

Brian R. Daniels, Terrence M. Dobrowsky, Edward M. Perkins, Sean X. Sun, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Specification of germline and somatic cell lineages in C. elegans originates in the polarized single-cell zygote. Several cell-fate determinants are partitioned unequally along the anterior-posterior axis of the zygote, ensuring the daughter cells a unique inheritance upon asymmetric cell division. Recent studies have revealed that partitioning of the germline determinant PIE-1 and the somatic determinant MEX-5 involve protein redistribution accompanied by spatiotemporal changes in protein diffusion rates. Here, we characterize the dynamics of MEX-5 in the zygote and propose a novel reaction/diffusion model to explain both its anterior enrichment and its remarkable intracellular dynamics without requiring asymmetrically distributed binding sites. We propose that asymmetric cortically localized PAR proteins mediate the anterior enrichment of MEX-5 by reversibly changing its diffusion rate at spatially distinct points in the embryo, thus generating a stable concentration gradient along the anteriorposterior axis of the cell. This work extends the scope of reaction/diffusion models to include not only germline morphogens, but also somatic determinants.

Original languageEnglish (US)
Pages (from-to)2579-2585
Number of pages7
JournalDevelopment
Volume137
Issue number15
DOIs
StatePublished - Aug 1 2010

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Zygote
Embryonic Structures
Asymmetric Cell Division
Proteins
Cell Lineage
Binding Sites

Keywords

  • C. Elegans
  • FCS
  • FRAP
  • Live-cell microscopy
  • MEX-5
  • Protein diffusion

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion. / Daniels, Brian R.; Dobrowsky, Terrence M.; Perkins, Edward M.; Sun, Sean X.; Wirtz, Denis.

In: Development, Vol. 137, No. 15, 01.08.2010, p. 2579-2585.

Research output: Contribution to journalArticle

Daniels, BR, Dobrowsky, TM, Perkins, EM, Sun, SX & Wirtz, D 2010, 'MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion', Development, vol. 137, no. 15, pp. 2579-2585. https://doi.org/10.1242/dev.051326
Daniels, Brian R. ; Dobrowsky, Terrence M. ; Perkins, Edward M. ; Sun, Sean X. ; Wirtz, Denis. / MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion. In: Development. 2010 ; Vol. 137, No. 15. pp. 2579-2585.
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