Methylcellulose cultures clotted and stained for permanent record

Y. McMahon, W. D. Hankins

Research output: Contribution to journalArticlepeer-review

Abstract

Whereas methylcellulose cultures have been valuable for biochemical studies on hemopoiesis in vitro, it has been difficult to study cellular morphology in these cultures. We have found that clotting factors can be added at any time during the culture period and non-adherent cells become entrapped in a semisolid clot. The clot can be removed, fixed and stained for detailed microscopic examination.

Original languageEnglish (US)
Pages (from-to)1081-1085
Number of pages5
JournalExperimental Hematology
Volume8
Issue number9
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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