Methylation-specific PCR unraveled

Sarah Derks; Marjolein H F M Lentjes; Debby M E I Hellebrekers; Adriaan P. De Bruïne; James G. Herman; Manon Van Engeland

Methylation-specific PCR unraveled

Sarah Derks, Marjolein H F M Lentjes, Debby M E I Hellebrekers, Adriaan P. De Bruïne, James G. Herman, Manon Van Engeland

School of Medicine

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

Original language	English (US)
Pages (from-to)	291-299
Number of pages	9
Journal	Cellular Oncology
Volume	26
Issue number	5-6
State	Published - 2004

Keywords

DNA methylation
Epigenetics
Methylation-specific PCR
Tumor marker
Tumor suppressor gene silencing

ASJC Scopus subject areas

Cell Biology
Pathology and Forensic Medicine
Oncology

Cite this

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title = "Methylation-specific PCR unraveled",

abstract = "Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.",

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AU - Derks, Sarah

AU - Lentjes, Marjolein H F M

AU - Hellebrekers, Debby M E I

AU - De Bruïne, Adriaan P.

AU - Herman, James G.

AU - Van Engeland, Manon

PY - 2004

Y1 - 2004

N2 - Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

AB - Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

KW - DNA methylation

KW - Epigenetics

KW - Methylation-specific PCR

KW - Tumor marker

KW - Tumor suppressor gene silencing

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Methylation-specific PCR unraveled

Abstract

Keywords

ASJC Scopus subject areas

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