Methylation SNaPshot: a method for the quantification of site-specific DNA methylation levels.

Zachary A Kaminsky, Arturas Petronis

Research output: Contribution to journalArticle

Abstract

As the role for epigenetic signals in genome regulation becomes increasingly understood, the ability to accurately measure levels of DNA methylation at individual cytosines throughout the genome is becoming increasingly important. In contrast to traditional methods for the quantification of cytosine methylation, such as cloning and sequencing of PCR fragments amplified from sodium bisulfite-modified DNA, recent developments have created a fast and effective alternative called methylation-sensitive single nucleotide primer extension (Ms-SNuPE). The following protocol outlines the steps necessary to design and perform Ms-SNuPE experiments using the SNaPshot chemistry and associated capillary electrophoresis platforms available through Applied Biosystems.

Original languageEnglish (US)
Pages (from-to)241-255
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume507
StatePublished - 2009
Externally publishedYes

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DNA Methylation
Methylation
Cytosine
Nucleotides
Genome
Capillary Electrophoresis
Epigenomics
Organism Cloning
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Methylation SNaPshot : a method for the quantification of site-specific DNA methylation levels. / Kaminsky, Zachary A; Petronis, Arturas.

In: Methods in molecular biology (Clifton, N.J.), Vol. 507, 2009, p. 241-255.

Research output: Contribution to journalArticle

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