Abstract
Objective: To test DNA methylation profiling in detection of urothethial carcinoma in urine. Study Design: Thirty-three bladder specimens were analyzed for the DNA methylation status of p14ARF, p16INK4a, RASSF1, APC, GSTP, E-Cad and CyclinD2 genes to determine if there is a difference in gene methylation between benign and malignant cases. Urine samples were analyzed in a feasibility study. Finally, methylation profiles of urine samples were obtained and compared with follow-up biopsy diagnoses. Results: We found methylated genes in 18% benign, 37% urothelial carcinoma in situ and 93% infiltrating urothelial carcinoma cases (p = 0.001). Methylation profiles from the 18 urine samples revealed a significantly higher prevalence of methylated genes in carcinoma cases than benign cases (100% vs. 50%, p = 0.025). We analyzed methylation profiles in 37 cytologically atypical urine samples with malignant or benign diagnosis on surgical follow-up and found that only APC (55% in malignant vs. 0% in benign, p = 0.025) and CyclinD2 were differentially methylated (35% in malignant vs. 0% in benign, p = 0.2) while p14ARF, p16INK4a, RASSF1, GSTP and E-Cad had similar methylation profiles. Conclusion: These results suggest that methylation of p14ARF, p16INK4a, RASSFl, GSTP and E-Cad genes may not accurately identify carcinoma, but methylated APC and CyclinD2 might be useful biomarkers for urothelial carcinoma in urine.
Original language | English (US) |
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Pages (from-to) | 499-506 |
Number of pages | 8 |
Journal | Acta cytologica |
Volume | 50 |
Issue number | 5 |
DOIs | |
State | Published - Jan 1 2006 |
Keywords
- Cancer
- Methylation
- Multiplex MSP
- Tumor suppressor genes
- Urothelial
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology