Methylation of the hypoxanthine phosphoribosyltransferase locus on the human X chromosome: Implications for X chromosome inactivation

S. F. Wolf, D. J. Jolly, K. D. Lunnen, T. Friedmann, B. R. Migeon

Research output: Contribution to journalArticle

Abstract

To explore the role of DNA methylation in maintaining dosage compensation of X chromosome-linked genes and in regulating the transcriptional activity of 'housekeeping' genes, we characterized methylation of active, inactive, and derepressed alleles at the locus for hypoxanthine phosphoribosyltransferase (HPRT) on the human X chromosome. The methylation of Hpa II and Hha I sites in HPRT alleles on the active X chromosome was the same in all tissues. The consensus pattern includes hypomethylation of 5' clustered sites and extensive methylation in the 3' sequence. The striking feature of methylation of inactive X-chromosome alleles is nonuniformity and less extensive hypomethylation of the 5' cluster. Analysis of HPRT alleles reactivated in response to 5-azacytidine showed at least partial restoration of the consensus pattern. These observations indicate that methylation or housekeeping genes on the X chromosome is the same as that of autosomal ones and that the overall pattern and methylation of multiple sites within a cluster may cooperate to facilitate transcription. Furthermore, the fidelity of methylation of the active allele and the extensive drift in methylation of the inactive allele suggest that mechanisms involved in X-chromosome dosage compensation may be directed at the active rather than inactive X-chromosome.

Original languageEnglish (US)
Pages (from-to)2806-2810
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number9 I
DOIs
StatePublished - Jan 1 1984

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this