Methyl-directed repair of frameshift heteroduplexes in cell extracts from Escherichia coli

B. A. Learn, R. H. Grafstrom

Research output: Contribution to journalArticlepeer-review

Abstract

The methyl-directed DNA repair efficiency of a series of M13mp9 frameshift heteroduplexes containing 1, 2, or 3 unpaired bases was determined by using an in vitro DNA mismatch repair assay. Repair of hemimethylated frameshift heteroduplexes in vitro was directed to the unmethylated strand; was dependent on MutH, MutL, and MutS; and was equally efficient on base insertions and deletions. However, fully methylated frameshift heteroduplexes were resistant to repair, while totally unmethylated substrates were repaired with no strand bias. Hemimethylated 1-, 2-, or 3-base insertion and deletion heteroduplexes were repaired by the methyl-directed mismatch repair pathway as efficiently as the G · T mismatch. These results are consistent with earlier in vivo studies and demonstrate the involvement of methyl-directed DNA repair in the efficient prevention of frameshift mutations.

Original languageEnglish (US)
Pages (from-to)6473-6481
Number of pages9
JournalJournal of bacteriology
Volume171
Issue number12
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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