TY - JOUR
T1 - Methoxychlor inhibits growth and induces atresia through the aryl hydrocarbon receptor pathway in mouse ovarian antral follicles
AU - Basavarajappa, Mallikarjuna S.
AU - Hernández-Ochoa, Isabel
AU - Wang, Wei
AU - Flaws, Jodi A.
PY - 2012/8
Y1 - 2012/8
N2 - Methoxychlor (MXC) is an organochlorine pesticide used against pests that attack crops, vegetables, and livestock. MXC inhibits growth and induces atresia (death) of mouse ovarian antral follicles in vitro. Since several studies indicate that many chemicals act through the aryl hydrocarbon receptor (AHR) pathway, the current study tested the hypothesis that MXC binds to the AHR to inhibit growth and induce atresia of antral follicles. The data indicate that MXC binds to AHR. Further, a relatively high dose of MXC (100μg/ml) inhibits growth and induces atresia in both wild-type (WT) and AHR null (AHRKO) follicles, whereas a lower dose of MXC (10μg/ml) inhibits growth and induces atresia in WT, but not in AHRKO follicles. These data indicate that AHR deletion partially protects antral follicles from MXC induced slow growth and atresia. Collectively, these data show that MXC may act through the AHR pathway to inhibit follicle growth and induce atresia in antral follicles of the ovary.
AB - Methoxychlor (MXC) is an organochlorine pesticide used against pests that attack crops, vegetables, and livestock. MXC inhibits growth and induces atresia (death) of mouse ovarian antral follicles in vitro. Since several studies indicate that many chemicals act through the aryl hydrocarbon receptor (AHR) pathway, the current study tested the hypothesis that MXC binds to the AHR to inhibit growth and induce atresia of antral follicles. The data indicate that MXC binds to AHR. Further, a relatively high dose of MXC (100μg/ml) inhibits growth and induces atresia in both wild-type (WT) and AHR null (AHRKO) follicles, whereas a lower dose of MXC (10μg/ml) inhibits growth and induces atresia in WT, but not in AHRKO follicles. These data indicate that AHR deletion partially protects antral follicles from MXC induced slow growth and atresia. Collectively, these data show that MXC may act through the AHR pathway to inhibit follicle growth and induce atresia in antral follicles of the ovary.
KW - Antral follicles
KW - Aryl hydrocarbon receptor
KW - Atresia
KW - Growth
KW - Methoxychlor
KW - Mouse
KW - Ovary
UR - http://www.scopus.com/inward/record.url?scp=84861679507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861679507&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2012.03.007
DO - 10.1016/j.reprotox.2012.03.007
M3 - Article
C2 - 22484361
AN - SCOPUS:84861679507
SN - 0890-6238
VL - 34
SP - 16
EP - 21
JO - Reproductive Toxicology
JF - Reproductive Toxicology
IS - 1
ER -