Methods to monitor cell surface expression and endocytic trafficking of CFTR in polarized epithelial cells.

Jennifer M. Bomberger, William B Guggino, Bruce A. Stanton

Research output: Contribution to journalArticle

Abstract

Cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride secretion is critical to maintaining airway surface hydration and efficient mucociliary clearance in the upper airways. Mutations in CFTR in cystic fibrosis lead to reduced expression of functional CFTR channels at the apical plasma membrane of the airway epithelium, leading to dehydration of the airway surface liquid and diminished mucociliary clearance. Cell surface CFTR is modulated by changes in CFTR endocytosis and recycling, effectively altering the cell surface abundance of the channel. This chapter examines current methods employed to measure the cell surface expression of CFTR, as well as methods to monitor CFTR movement through the endocytic pathway.

Original languageEnglish (US)
Pages (from-to)271-283
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume741
DOIs
StatePublished - 2011
Externally publishedYes

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Cystic Fibrosis Transmembrane Conductance Regulator
Epithelial Cells
Mucociliary Clearance
Recycling
Endocytosis
Dehydration
Cystic Fibrosis
Chlorides
Epithelium
Cell Membrane
Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Methods to monitor cell surface expression and endocytic trafficking of CFTR in polarized epithelial cells. / Bomberger, Jennifer M.; Guggino, William B; Stanton, Bruce A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 741, 2011, p. 271-283.

Research output: Contribution to journalArticle

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