Methods in laboratory investigation. An evaluation of methods to quantitate the chick chorioallantoic membrane assay in angiogenesis

M. T. Vu, C. F. Smith, P. C. Burger, G. K. Klintworth

Research output: Contribution to journalArticle

Abstract

The vascular responses by chick chorioallantoic membranes (CAM) to more than 150 normal and chemically injured rat corneas grafted to shell-less chicken CAMs were evaluated independently by three observers in a masked fashion by in vivo stereomicroscopy, projections of colored transparencies, and by light microscopy of tissue sections of the grafts. The experience gained from this study is reviewed as a point of focus for the strengths and weaknesses of the CAM technique in the assay of potential angiogenic substances. Despite certain shortcomings, the CAM technique can provide useful information relevant to studies on angiogenesis, particularly when the subjective CAM method is supplemented by histological evaluation of grafted tissues.

Original languageEnglish (US)
Pages (from-to)499-508
Number of pages10
JournalLaboratory Investigation
Volume53
Issue number4
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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