Methods for swath™: Data independent acquisition on tripletof mass spectrometers

Ronald J. Holewinski, Sarah J. Parker, Andrea D. Matlock, Vidya Venkatraman, Jennifer E. Van Eyk

Research output: Contribution to journalArticlepeer-review

Abstract

Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex Triple TOF mass spectrometers and data analysis using the Sciex processing software.

Original languageEnglish (US)
Pages (from-to)265-279
Number of pages15
JournalMethods in Molecular Biology
Volume1410
DOIs
StatePublished - Feb 1 2016

Keywords

  • Data independent acquisition (DIA)
  • Mass spectrometry
  • Quantitative proteomics
  • Spectral ion library
  • Swath

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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