Methods for studying microRNA functions during stress

Yoshinari Ando; Anthony K.L. Leung

doi:10.1007/978-1-4939-2522-3_9

Methods for studying microRNA functions during stress

Yoshinari Ando, Anthony K.L. Leung

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Constituting 5% of the human genome, microRNAs represent a sizeable class of gene regulators that is predicted to control the expression of at least 60% of all protein-coding RNAs. Dysregulation of microRNA function results in developmental defects and pathological diseases such as cancers and neurological disorders. Intriguingly, many phenotypes of microRNA deficiencies are subdued in normal condition but manifested apparently upon stress. Here, we outline experimental methods to monitor the level, targets, and activity of microRNAs as the first few steps to characterize how microRNA functions are altered upon stress.

Original language	English (US)
Pages (from-to)	115
Number of pages	1
Journal	Methods in Molecular Biology
Volume	1292
DOIs	https://doi.org/10.1007/978-1-4939-2522-3_9
State	Published - 2015

Keywords

Argonaute
CLIP-seq
Dual-luciferase reporter assay
MicroRNA
Real-time PCR

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2522-3_9

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AB - Constituting 5% of the human genome, microRNAs represent a sizeable class of gene regulators that is predicted to control the expression of at least 60% of all protein-coding RNAs. Dysregulation of microRNA function results in developmental defects and pathological diseases such as cancers and neurological disorders. Intriguingly, many phenotypes of microRNA deficiencies are subdued in normal condition but manifested apparently upon stress. Here, we outline experimental methods to monitor the level, targets, and activity of microRNAs as the first few steps to characterize how microRNA functions are altered upon stress.

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KW - Dual-luciferase reporter assay

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KW - Real-time PCR

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JO - Methods in Molecular Biology

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Methods for studying microRNA functions during stress

Abstract

Keywords

ASJC Scopus subject areas

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