Method to determine the number of bacterial spores within aerosol particles

Monica Carrera, Jana Kesavan, Ruben Zandomeni, Jose Luis Sagripanti

Research output: Contribution to journalArticle

Abstract

We describe methodology to reveal the number of microbial spores within aerosol particles. The procedure involves visualization under differential- interference-contrast microscopy enhanced by high-resolution photography and further analysis by computer-assisted imaging. The method was used to analyze spore of Bacillus globigii in aerosols generated by a small (pressured metered-dose inhaler type) generator. Particles consisting in 1 or 2 spores accounted for 85% of all generated particles. This percentage rose to 91% when the same aerosol was collected on an Andersen cascade impactor that collected particles larger than 0.65 μm and was even higher (96%) when particles larger than 3.3 μm were also eliminated. These results demonstrate that the imaging analysis of aerosol particles collected on glass slides is sensitive to even relatively small changes in aerosol particle composition. The accuracy of the enhanced microscopic method described herein (differences between visual and computer analysis were approximately 3% of the total particle counts) seems adequate to determine the spore composition of aerosols of interest in biodefense.

Original languageEnglish (US)
Pages (from-to)960-965
Number of pages6
JournalAerosol Science and Technology
Volume39
Issue number10
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Aerosols
Particles (particulate matter)
spore
aerosol
Imaging techniques
Photography
Bacilli
photography
Chemical analysis
visualization
method
microscopy
Microscopic examination
Visualization
glass
particle
Glass
methodology
analysis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Mechanical Engineering

Cite this

Method to determine the number of bacterial spores within aerosol particles. / Carrera, Monica; Kesavan, Jana; Zandomeni, Ruben; Sagripanti, Jose Luis.

In: Aerosol Science and Technology, Vol. 39, No. 10, 10.2005, p. 960-965.

Research output: Contribution to journalArticle

Carrera, Monica ; Kesavan, Jana ; Zandomeni, Ruben ; Sagripanti, Jose Luis. / Method to determine the number of bacterial spores within aerosol particles. In: Aerosol Science and Technology. 2005 ; Vol. 39, No. 10. pp. 960-965.
@article{03e83b716cb44065922c7d2731ab744b,
title = "Method to determine the number of bacterial spores within aerosol particles",
abstract = "We describe methodology to reveal the number of microbial spores within aerosol particles. The procedure involves visualization under differential- interference-contrast microscopy enhanced by high-resolution photography and further analysis by computer-assisted imaging. The method was used to analyze spore of Bacillus globigii in aerosols generated by a small (pressured metered-dose inhaler type) generator. Particles consisting in 1 or 2 spores accounted for 85{\%} of all generated particles. This percentage rose to 91{\%} when the same aerosol was collected on an Andersen cascade impactor that collected particles larger than 0.65 μm and was even higher (96{\%}) when particles larger than 3.3 μm were also eliminated. These results demonstrate that the imaging analysis of aerosol particles collected on glass slides is sensitive to even relatively small changes in aerosol particle composition. The accuracy of the enhanced microscopic method described herein (differences between visual and computer analysis were approximately 3{\%} of the total particle counts) seems adequate to determine the spore composition of aerosols of interest in biodefense.",
author = "Monica Carrera and Jana Kesavan and Ruben Zandomeni and Sagripanti, {Jose Luis}",
year = "2005",
month = "10",
doi = "10.1080/02786820500352098",
language = "English (US)",
volume = "39",
pages = "960--965",
journal = "Aerosol Science and Technology",
issn = "0278-6826",
publisher = "Taylor and Francis Ltd.",
number = "10",

}

TY - JOUR

T1 - Method to determine the number of bacterial spores within aerosol particles

AU - Carrera, Monica

AU - Kesavan, Jana

AU - Zandomeni, Ruben

AU - Sagripanti, Jose Luis

PY - 2005/10

Y1 - 2005/10

N2 - We describe methodology to reveal the number of microbial spores within aerosol particles. The procedure involves visualization under differential- interference-contrast microscopy enhanced by high-resolution photography and further analysis by computer-assisted imaging. The method was used to analyze spore of Bacillus globigii in aerosols generated by a small (pressured metered-dose inhaler type) generator. Particles consisting in 1 or 2 spores accounted for 85% of all generated particles. This percentage rose to 91% when the same aerosol was collected on an Andersen cascade impactor that collected particles larger than 0.65 μm and was even higher (96%) when particles larger than 3.3 μm were also eliminated. These results demonstrate that the imaging analysis of aerosol particles collected on glass slides is sensitive to even relatively small changes in aerosol particle composition. The accuracy of the enhanced microscopic method described herein (differences between visual and computer analysis were approximately 3% of the total particle counts) seems adequate to determine the spore composition of aerosols of interest in biodefense.

AB - We describe methodology to reveal the number of microbial spores within aerosol particles. The procedure involves visualization under differential- interference-contrast microscopy enhanced by high-resolution photography and further analysis by computer-assisted imaging. The method was used to analyze spore of Bacillus globigii in aerosols generated by a small (pressured metered-dose inhaler type) generator. Particles consisting in 1 or 2 spores accounted for 85% of all generated particles. This percentage rose to 91% when the same aerosol was collected on an Andersen cascade impactor that collected particles larger than 0.65 μm and was even higher (96%) when particles larger than 3.3 μm were also eliminated. These results demonstrate that the imaging analysis of aerosol particles collected on glass slides is sensitive to even relatively small changes in aerosol particle composition. The accuracy of the enhanced microscopic method described herein (differences between visual and computer analysis were approximately 3% of the total particle counts) seems adequate to determine the spore composition of aerosols of interest in biodefense.

UR - http://www.scopus.com/inward/record.url?scp=29644431860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29644431860&partnerID=8YFLogxK

U2 - 10.1080/02786820500352098

DO - 10.1080/02786820500352098

M3 - Article

AN - SCOPUS:29644431860

VL - 39

SP - 960

EP - 965

JO - Aerosol Science and Technology

JF - Aerosol Science and Technology

SN - 0278-6826

IS - 10

ER -