Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)

K. Sandberg, R. L. Schnaar, J. T. Coyle

Research output: Contribution to journalArticle

Abstract

Monoethylcholine aziridinium ion (AF64A), which is generated from the precursor, acetylcholine mustard hydrochloride, exerts selective neurotoxic effects against brain cholinergic neurons when injected intracerebrally. Conditions associated with optimal generation of AF64A were examined. The results indicate that hydrolysis and cyclization of the precursor are optimal at 25°C with the former occurring at pH 9.0 and the latter at pH 7.3. The aziridinium ion is best stabilized at pH 7.3 at 4°C.

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalJournal of Neuroscience Methods
Volume14
Issue number2
DOIs
StatePublished - Jul 1985

Keywords

  • AF64A
  • cholinergic
  • methyl choline mustard aziridinium ion
  • neurotoxin
  • nitrogen mustard

ASJC Scopus subject areas

  • Neuroscience(all)

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