Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)

K. Sandberg; R. L. Schnaar; J. T. Coyle

doi:10.1016/0165-0270(85)90127-X

Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)

K. Sandberg, R. L. Schnaar, J. T. Coyle

School of Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Monoethylcholine aziridinium ion (AF64A), which is generated from the precursor, acetylcholine mustard hydrochloride, exerts selective neurotoxic effects against brain cholinergic neurons when injected intracerebrally. Conditions associated with optimal generation of AF64A were examined. The results indicate that hydrolysis and cyclization of the precursor are optimal at 25°C with the former occurring at pH 9.0 and the latter at pH 7.3. The aziridinium ion is best stabilized at pH 7.3 at 4°C.

Original language	English (US)
Pages (from-to)	143-148
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/0165-0270(85)90127-X
State	Published - Jul 1985

Keywords

AF64A
cholinergic
methyl choline mustard aziridinium ion
neurotoxin
nitrogen mustard

ASJC Scopus subject areas

General Neuroscience

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10.1016/0165-0270(85)90127-X

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title = "Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)",

abstract = "Monoethylcholine aziridinium ion (AF64A), which is generated from the precursor, acetylcholine mustard hydrochloride, exerts selective neurotoxic effects against brain cholinergic neurons when injected intracerebrally. Conditions associated with optimal generation of AF64A were examined. The results indicate that hydrolysis and cyclization of the precursor are optimal at 25°C with the former occurring at pH 9.0 and the latter at pH 7.3. The aziridinium ion is best stabilized at pH 7.3 at 4°C.",

keywords = "AF64A, cholinergic, methyl choline mustard aziridinium ion, neurotoxin, nitrogen mustard",

author = "K. Sandberg and Schnaar, {R. L.} and Coyle, {J. T.}",

year = "1985",

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T1 - Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)

AU - Sandberg, K.

AU - Schnaar, R. L.

AU - Coyle, J. T.

PY - 1985/7

Y1 - 1985/7

N2 - Monoethylcholine aziridinium ion (AF64A), which is generated from the precursor, acetylcholine mustard hydrochloride, exerts selective neurotoxic effects against brain cholinergic neurons when injected intracerebrally. Conditions associated with optimal generation of AF64A were examined. The results indicate that hydrolysis and cyclization of the precursor are optimal at 25°C with the former occurring at pH 9.0 and the latter at pH 7.3. The aziridinium ion is best stabilized at pH 7.3 at 4°C.

AB - Monoethylcholine aziridinium ion (AF64A), which is generated from the precursor, acetylcholine mustard hydrochloride, exerts selective neurotoxic effects against brain cholinergic neurons when injected intracerebrally. Conditions associated with optimal generation of AF64A were examined. The results indicate that hydrolysis and cyclization of the precursor are optimal at 25°C with the former occurring at pH 9.0 and the latter at pH 7.3. The aziridinium ion is best stabilized at pH 7.3 at 4°C.

KW - AF64A

KW - cholinergic

KW - methyl choline mustard aziridinium ion

KW - neurotoxin

KW - nitrogen mustard

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Method for the quantitation and characterization of the cholinergic neurotoxin, monoethylcholine mustard aziridinium ion (AF64A)

Abstract

Keywords

ASJC Scopus subject areas

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