Method for measuring mRNA decay rate in saccharomyces cerevisiae

Wenqian Hu, Jeff Coller

Research output: Chapter in Book/Report/Conference proceedingChapter


Eukaryotic mRNA degradation is an essential aspect of gene regulation. Properly turning off transcript generation ensures that protein synthesis does not occur indefinitely. By ensuring that all mRNAs are destroyed, cells can adapt quickly to changing physiological and environmental conditions. Eukaryotic cytoplasmic mRNA degradation is predominately initiated by removal of the poly(A) tail at the 3′ end (deadenylation). Following deadenylation, either the mRNA is degraded in a 3′-5′ manner or the cap is removed and the mRNA is degraded 5′-3′ (reviewed in Coller and Parker, 2004). Determining mRNA decay rates, as indicated by mRNA half-life, is vital to understand how mRNA stability is modulated under various physiological conditions.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Number of pages19
ISBN (Print)9780124200371
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Formaldehyde agarose denaturing gel electrophoresis
  • mRNA decay rate
  • mRNA half-life calculation
  • Northern transfer
  • Radiolabeled oligonucleotide probe
  • Yeast cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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