TY - JOUR
T1 - Methacrylated gellan gum and hyaluronic acid hydrogel blends for image-guided neurointerventions
AU - Vieira, Sílvia
AU - Strymecka, Paulina
AU - Stanaszek, Luiza
AU - Silva-Correia, Joana
AU - Drela, Katarzyna
AU - Fiedorowicz, Michał
AU - Malysz-Cymborska, Izabela
AU - Rogujski, Piotr
AU - Janowski, Miroslaw
AU - Reis, Rui L.
AU - Lukomska, Barbara
AU - Walczak, Piotr
AU - Oliveira, J. Miguel
N1 - Funding Information:
Sílvia Vieira acknowledges the FCT PhD scholarship (SFRH/BD/ 102710/2014). The FCT distinction attributed to J. Miguel Oli-veira under the Investigator FCT program (IF/01285/2015) and J. Silva-Correia (IF/00115/2015) are also greatly acknowledged. The authors also acknowledge the funds provided under the project NanoTech4ALS, funded under the EU FP7 M-ERA.NET program, and ESF (POWR.03.02.00-00-I028/17-00).
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2020/7/21
Y1 - 2020/7/21
N2 - Cell-based therapies delivered via intrathecal injection are considered as one of the most promising solutions for the treatment of amyotrophic lateral sclerosis (ALS). Herein, injectable manganese-based biocompatible hydrogel blends were developed, that can allow image-guided cell delivery. The hydrogels can also provide physical support for cells during injection, and at the intrathecal space after transplantation, while assuring cell survival. In this regard, different formulations of methacrylated gellan gum/hyaluronic acid hydrogel blends (GG-MA/HA) were considered as a vehicle for cell delivery. The hydrogels blends were supplemented with paramagnetic Mn2+ to allow a real-time monitorization of hydrogel deposition via T1-weighted magnetic resonance imaging (MRI). The developed hydrogels were easily extruded and formed a stable fiber upon injection into the cerebrospinal fluid. Hydrogels prepared with a 75 : 25 GG-MA to HA ratio supplemented with MnCl2 at 0.1 mM showed controlled hydrogel degradation, suitable permeability, and a distinct MRI signal in vitro and in vivo. Additionally, human-derived adipose stem cells encapsulated in 75 : 25 GG-MA/HA hydrogels remained viable for up to 14 days of culture in vitro. Therefore, the engineered hydrogels can be an excellent tool for injectable image-guided cell delivery approaches.
AB - Cell-based therapies delivered via intrathecal injection are considered as one of the most promising solutions for the treatment of amyotrophic lateral sclerosis (ALS). Herein, injectable manganese-based biocompatible hydrogel blends were developed, that can allow image-guided cell delivery. The hydrogels can also provide physical support for cells during injection, and at the intrathecal space after transplantation, while assuring cell survival. In this regard, different formulations of methacrylated gellan gum/hyaluronic acid hydrogel blends (GG-MA/HA) were considered as a vehicle for cell delivery. The hydrogels blends were supplemented with paramagnetic Mn2+ to allow a real-time monitorization of hydrogel deposition via T1-weighted magnetic resonance imaging (MRI). The developed hydrogels were easily extruded and formed a stable fiber upon injection into the cerebrospinal fluid. Hydrogels prepared with a 75 : 25 GG-MA to HA ratio supplemented with MnCl2 at 0.1 mM showed controlled hydrogel degradation, suitable permeability, and a distinct MRI signal in vitro and in vivo. Additionally, human-derived adipose stem cells encapsulated in 75 : 25 GG-MA/HA hydrogels remained viable for up to 14 days of culture in vitro. Therefore, the engineered hydrogels can be an excellent tool for injectable image-guided cell delivery approaches.
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U2 - 10.1039/d0tb00877j
DO - 10.1039/d0tb00877j
M3 - Article
C2 - 32542280
AN - SCOPUS:85088091221
SN - 2050-7518
VL - 8
SP - 5928
EP - 5937
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 27
ER -