Metastatic Human Rhabdomyosarcoma: Molecular/Cellular and Cytogenetic Analysis of a Novel Cellular Model

Thea Kalebie, Jean Gabriel Judde, Maria Velez-Yanguas, Turid Knutsen, Lee J. Helman

Research output: Contribution to journalArticle

Abstract

A new human metastatic rhabdomyosarcoma (RMS) model was established and analyzed for a number of biologic, cytogenetic and molecular parameters. Consistent with previous studies, the metastatic capacity of different RMS cell variants did not correlate with their tumorigenic or proliferative capacities. Interestingly, a highly metastatic variant was diploid, while a nonmetastatic variant was tetraploid, which parallels previous clinical observations. Genes whose expression had been found to be associated with either low-or high-metastatic capacity in carcinoma or melanoma did not show a similar association with different metastatic variants of RMS, derived from a mesenchymal tumor. We also found, in transient reporter gene assays, that several promoters had higher transcriptional activity in highly metastatic than in nonmetastatic RMS cell variants. This novel human RMS metastatic model may be instrumental for a better understanding of the regulatory pathways that control the metastatic phenotype of tumors of mesenchymal origin.

Original languageEnglish (US)
Pages (from-to)83-96
Number of pages14
JournalInvasion and Metastasis
Volume16
Issue number2
StatePublished - Dec 1 1996

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Keywords

  • Rhabdomyosarcoma

ASJC Scopus subject areas

  • Cancer Research

Cite this

Kalebie, T., Judde, J. G., Velez-Yanguas, M., Knutsen, T., & Helman, L. J. (1996). Metastatic Human Rhabdomyosarcoma: Molecular/Cellular and Cytogenetic Analysis of a Novel Cellular Model. Invasion and Metastasis, 16(2), 83-96.