Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes

Lieping Chen, Stephanie Ashe, Mitra C. Singhal, Denise A. Galloway, Ingegerd Hellström, Karl Erik Hellström

Research output: Contribution to journalArticle

Abstract

The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression of either of the viral genes from HPV-16 made the cells metastatic in nude mice. In contrast, expression of the E6 and E7 genes of HPV type 6 (HPV-6b), which is frequently found in nonmalignant HPV-associated diseases, did not. The metastatic ability of cells transduced with viral genes of HPV-16 did not correlate with their growth rate or sensitivity to destruction by natural killer cells. Our results demonstrate that expression of oncogenic proteins of HPV-16 can cause tumor metastasis and implicate HPV-16 in an important role regarding the progression of HPV-associated human cancers.

Original languageEnglish (US)
Pages (from-to)6523-6527
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number14
StatePublished - Jul 15 1993
Externally publishedYes

Fingerprint

Human papillomavirus 16
Genes
Viral Genes
Neoplasms
DNA Tumor Viruses
Neoplasm Metastasis
Human papillomavirus 6
Oncogene Proteins
Viral DNA
Retroviridae
Nude Mice
Natural Killer Cells
Carcinoma
Cell Line
Growth

Keywords

  • Cancer metastasis
  • Malignancy
  • Transformation
  • Tumorigenicity

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Chen, L., Ashe, S., Singhal, M. C., Galloway, D. A., Hellström, I., & Hellström, K. E. (1993). Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes. Proceedings of the National Academy of Sciences of the United States of America, 90(14), 6523-6527.

Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes. / Chen, Lieping; Ashe, Stephanie; Singhal, Mitra C.; Galloway, Denise A.; Hellström, Ingegerd; Hellström, Karl Erik.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 14, 15.07.1993, p. 6523-6527.

Research output: Contribution to journalArticle

Chen, L, Ashe, S, Singhal, MC, Galloway, DA, Hellström, I & Hellström, KE 1993, 'Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 14, pp. 6523-6527.
Chen, Lieping ; Ashe, Stephanie ; Singhal, Mitra C. ; Galloway, Denise A. ; Hellström, Ingegerd ; Hellström, Karl Erik. / Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 14. pp. 6523-6527.
@article{70b23cb386cd47c0aaa3c31aed2a212f,
title = "Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes",
abstract = "The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression of either of the viral genes from HPV-16 made the cells metastatic in nude mice. In contrast, expression of the E6 and E7 genes of HPV type 6 (HPV-6b), which is frequently found in nonmalignant HPV-associated diseases, did not. The metastatic ability of cells transduced with viral genes of HPV-16 did not correlate with their growth rate or sensitivity to destruction by natural killer cells. Our results demonstrate that expression of oncogenic proteins of HPV-16 can cause tumor metastasis and implicate HPV-16 in an important role regarding the progression of HPV-associated human cancers.",
keywords = "Cancer metastasis, Malignancy, Transformation, Tumorigenicity",
author = "Lieping Chen and Stephanie Ashe and Singhal, {Mitra C.} and Galloway, {Denise A.} and Ingegerd Hellstr{\"o}m and Hellstr{\"o}m, {Karl Erik}",
year = "1993",
month = "7",
day = "15",
language = "English (US)",
volume = "90",
pages = "6523--6527",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "14",

}

TY - JOUR

T1 - Metastatic conversion of cells by expression of human papillomavirus type 16 E6 and E7 genes

AU - Chen, Lieping

AU - Ashe, Stephanie

AU - Singhal, Mitra C.

AU - Galloway, Denise A.

AU - Hellström, Ingegerd

AU - Hellström, Karl Erik

PY - 1993/7/15

Y1 - 1993/7/15

N2 - The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression of either of the viral genes from HPV-16 made the cells metastatic in nude mice. In contrast, expression of the E6 and E7 genes of HPV type 6 (HPV-6b), which is frequently found in nonmalignant HPV-associated diseases, did not. The metastatic ability of cells transduced with viral genes of HPV-16 did not correlate with their growth rate or sensitivity to destruction by natural killer cells. Our results demonstrate that expression of oncogenic proteins of HPV-16 can cause tumor metastasis and implicate HPV-16 in an important role regarding the progression of HPV-associated human cancers.

AB - The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression of either of the viral genes from HPV-16 made the cells metastatic in nude mice. In contrast, expression of the E6 and E7 genes of HPV type 6 (HPV-6b), which is frequently found in nonmalignant HPV-associated diseases, did not. The metastatic ability of cells transduced with viral genes of HPV-16 did not correlate with their growth rate or sensitivity to destruction by natural killer cells. Our results demonstrate that expression of oncogenic proteins of HPV-16 can cause tumor metastasis and implicate HPV-16 in an important role regarding the progression of HPV-associated human cancers.

KW - Cancer metastasis

KW - Malignancy

KW - Transformation

KW - Tumorigenicity

UR - http://www.scopus.com/inward/record.url?scp=0027197501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027197501&partnerID=8YFLogxK

M3 - Article

C2 - 8341664

AN - SCOPUS:0027197501

VL - 90

SP - 6523

EP - 6527

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 14

ER -