Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans

A. Golden, P. L. Sadler, M. R. Wallenfang, J. M. Schumacher, D. R. Hamill, G. Bates, B. Bowerman, Geraldine Seydoux, D. C. Shakes

Research output: Contribution to journalArticle

Abstract

The metaphase to anaphase transition is a critical stage of the eukaryotic cell cycle, and, thus, it is highly regulated. Errors during this transition can lead to chromosome segregation defects and death of the organism. In genetic screens for temperature-sensitive maternal effect embryonic lethal (Mel) mutants, we have identified 32 mutants in the nematode Caenorhabditis elegans in which fertilized embryos arrest as one-cell embryos. In these mutant embryos, the oocyte chromosomes arrest in metaphase of meiosis I without transitioning to anaphase or producing polar bodies. An additional block in M phase exit is evidenced by the failure to form pronuclei and the persistence of phosphohistone H3 and MPM-2 antibody staining. Spermatocyte meiosis is also perturbed; primary spermatocytes arrest in metaphase of meiosis I and fail to produce secondary spermatocytes. Analogous mitotic defects cause M phase delays in mitotic germline proliferation. We have named this class of mutants "mat" for metaphase to anaphase transition defective. These mutants, representing six different complementation groups, all map near genes that encode subunits of the anaphase promoting complex or cyclosome, and, here, we show that one of the genes, emb-27, encodes the C. elegans CDC16 ortholog.

Original languageEnglish (US)
Pages (from-to)1469-1482
Number of pages14
JournalJournal of Cell Biology
Volume151
Issue number7
DOIs
StatePublished - Dec 31 2000
Externally publishedYes

Fingerprint

Anaphase
Caenorhabditis elegans
Metaphase
Spermatocytes
Meiosis
Embryonic Structures
Cell Division
Anaphase-Promoting Complex-Cyclosome
Polar Bodies
Chromosome Segregation
Eukaryotic Cells
Genes
Oocytes
Cell Cycle
Chromosomes
Staining and Labeling
Temperature
Antibodies

Keywords

  • Anaphase promoting complex
  • C. elegans
  • Cell cycle
  • Meiosis
  • Metaphase arrest

ASJC Scopus subject areas

  • Cell Biology

Cite this

Golden, A., Sadler, P. L., Wallenfang, M. R., Schumacher, J. M., Hamill, D. R., Bates, G., ... Shakes, D. C. (2000). Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans. Journal of Cell Biology, 151(7), 1469-1482. https://doi.org/10.1083/jcb.151.7.1469

Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans. / Golden, A.; Sadler, P. L.; Wallenfang, M. R.; Schumacher, J. M.; Hamill, D. R.; Bates, G.; Bowerman, B.; Seydoux, Geraldine; Shakes, D. C.

In: Journal of Cell Biology, Vol. 151, No. 7, 31.12.2000, p. 1469-1482.

Research output: Contribution to journalArticle

Golden, A, Sadler, PL, Wallenfang, MR, Schumacher, JM, Hamill, DR, Bates, G, Bowerman, B, Seydoux, G & Shakes, DC 2000, 'Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans', Journal of Cell Biology, vol. 151, no. 7, pp. 1469-1482. https://doi.org/10.1083/jcb.151.7.1469
Golden A, Sadler PL, Wallenfang MR, Schumacher JM, Hamill DR, Bates G et al. Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans. Journal of Cell Biology. 2000 Dec 31;151(7):1469-1482. https://doi.org/10.1083/jcb.151.7.1469
Golden, A. ; Sadler, P. L. ; Wallenfang, M. R. ; Schumacher, J. M. ; Hamill, D. R. ; Bates, G. ; Bowerman, B. ; Seydoux, Geraldine ; Shakes, D. C. / Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans. In: Journal of Cell Biology. 2000 ; Vol. 151, No. 7. pp. 1469-1482.
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AU - Hamill, D. R.

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