Metallothionein gene expression in peripheral lymphocytes and renal dysfunction in a population environmentally exposed to cadmium

Jian Lu; Taiyi Jin; Gunnar Nordberg; Monica Nordberg

doi:10.1016/j.taap.2004.12.015

Metallothionein gene expression in peripheral lymphocytes and renal dysfunction in a population environmentally exposed to cadmium

Jian Lu, Taiyi Jin, Gunnar Nordberg, Monica Nordberg

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

In order to study the validity of metallothionein (MT) gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility to renal dysfunction, MT mRNA levels were measured using reverse transcription polymerase chain reaction (RT-PCR) in PBLs from residents living in a cadmium-contaminated area. MT mRNA levels were found to increase with the increase of blood cadmium (BCd) and urinary cadmium (UCd) levels. Basal MT mRNA levels were significantly correlated with the logarithm of BCd levels and the logarithm of UCd levels confirming that MT expression in PBLs is a biomarker of cadmium exposure and internal dose. An inverse relationship was observed between in vitro induced MT-mRNA level in PBLs and urinary N-acetyl-β-d- glucosaminidase (UNAG) suggesting that MT gene expression in PBLs may be used as a biomarker of susceptibility to renal toxicity of cadmium.

Original language	English (US)
Pages (from-to)	150-156
Number of pages	7
Journal	Toxicology and Applied Pharmacology
Volume	206
Issue number	2
DOIs	https://doi.org/10.1016/j.taap.2004.12.015
State	Published - Aug 15 2005

Keywords

Blood
Cadmium exposure
Gene expression
Human
Metallothionein
NAG
Peripheral lymphocytes
Renal dysfunction
Urine

ASJC Scopus subject areas

Toxicology
Pharmacology

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10.1016/j.taap.2004.12.015

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title = "Metallothionein gene expression in peripheral lymphocytes and renal dysfunction in a population environmentally exposed to cadmium",

abstract = "In order to study the validity of metallothionein (MT) gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility to renal dysfunction, MT mRNA levels were measured using reverse transcription polymerase chain reaction (RT-PCR) in PBLs from residents living in a cadmium-contaminated area. MT mRNA levels were found to increase with the increase of blood cadmium (BCd) and urinary cadmium (UCd) levels. Basal MT mRNA levels were significantly correlated with the logarithm of BCd levels and the logarithm of UCd levels confirming that MT expression in PBLs is a biomarker of cadmium exposure and internal dose. An inverse relationship was observed between in vitro induced MT-mRNA level in PBLs and urinary N-acetyl-β-d- glucosaminidase (UNAG) suggesting that MT gene expression in PBLs may be used as a biomarker of susceptibility to renal toxicity of cadmium.",

keywords = "Blood, Cadmium exposure, Gene expression, Human, Metallothionein, NAG, Peripheral lymphocytes, Renal dysfunction, Urine",

author = "Jian Lu and Taiyi Jin and Gunnar Nordberg and Monica Nordberg",

note = "Funding Information: Supported by European Union (ERB 3514 PL 971430), Swedish Agency for Research and Educational Cooperation with Developing Countries (SWE-94-147, SWE-96-173 and SAREC-1997-0485), Karolinska Institutet, China National Key Basic Research and Development Program 2002CB512905. Statistical testing of differences in Fig. 1 was performed by Dr Leif Nilsson, Department Mathematical Statistics, Umea University. ",

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AU - Lu, Jian

AU - Jin, Taiyi

AU - Nordberg, Gunnar

AU - Nordberg, Monica

N1 - Funding Information: Supported by European Union (ERB 3514 PL 971430), Swedish Agency for Research and Educational Cooperation with Developing Countries (SWE-94-147, SWE-96-173 and SAREC-1997-0485), Karolinska Institutet, China National Key Basic Research and Development Program 2002CB512905. Statistical testing of differences in Fig. 1 was performed by Dr Leif Nilsson, Department Mathematical Statistics, Umea University.

PY - 2005/8/15

Y1 - 2005/8/15

N2 - In order to study the validity of metallothionein (MT) gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility to renal dysfunction, MT mRNA levels were measured using reverse transcription polymerase chain reaction (RT-PCR) in PBLs from residents living in a cadmium-contaminated area. MT mRNA levels were found to increase with the increase of blood cadmium (BCd) and urinary cadmium (UCd) levels. Basal MT mRNA levels were significantly correlated with the logarithm of BCd levels and the logarithm of UCd levels confirming that MT expression in PBLs is a biomarker of cadmium exposure and internal dose. An inverse relationship was observed between in vitro induced MT-mRNA level in PBLs and urinary N-acetyl-β-d- glucosaminidase (UNAG) suggesting that MT gene expression in PBLs may be used as a biomarker of susceptibility to renal toxicity of cadmium.

AB - In order to study the validity of metallothionein (MT) gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility to renal dysfunction, MT mRNA levels were measured using reverse transcription polymerase chain reaction (RT-PCR) in PBLs from residents living in a cadmium-contaminated area. MT mRNA levels were found to increase with the increase of blood cadmium (BCd) and urinary cadmium (UCd) levels. Basal MT mRNA levels were significantly correlated with the logarithm of BCd levels and the logarithm of UCd levels confirming that MT expression in PBLs is a biomarker of cadmium exposure and internal dose. An inverse relationship was observed between in vitro induced MT-mRNA level in PBLs and urinary N-acetyl-β-d- glucosaminidase (UNAG) suggesting that MT gene expression in PBLs may be used as a biomarker of susceptibility to renal toxicity of cadmium.

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KW - Renal dysfunction

KW - Urine

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Metallothionein gene expression in peripheral lymphocytes and renal dysfunction in a population environmentally exposed to cadmium

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