Metal Ion Dependence of Cooperative Collapse Transitions in RNA

Sarvin Moghaddam, Gokhan Caliskan, Seema Chauhan, Changbong Hyeon, R. M. Briber, D. Thirumalai, Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

Positively charged counterions drive RNA molecules into compact configurations that lead to their biologically active structures. To understand how the valence and size of the cations influences the collapse transition in RNA, small-angle X-ray scattering was used to follow the decrease in the radius of gyration (Rg) of the Azoarcus and Tetrahymena ribozymes in different cations. Small, multivalent cations induced the collapse of both ribozymes more efficiently than did monovalent ions. Thus, the cooperativity of the collapse transition depends on the counterion charge density. Singular value decomposition of the scattering curves showed that folding of the smaller and more thermostable Azoarcus ribozyme is well described by two components, whereas collapse of the larger Tetrahymena ribozyme involves at least one intermediate. The ion-dependent persistence length, extracted from the distance distribution of the scattering vectors, shows that the Azoarcus ribozyme is less flexible at the midpoint of transition in low-charge-density ions than in high-charge-density ions. We conclude that the formation of sequence-specific tertiary interactions in the Azoarcus ribozyme overlaps with neutralization of the phosphate charge, while tertiary folding of the Tetrahymena ribozyme requires additional counterions. Thus, the stability of the RNA structure determines its sensitivity to the valence and size of the counterions.

Original languageEnglish (US)
Pages (from-to)753-764
Number of pages12
JournalJournal of molecular biology
Volume393
Issue number3
DOIs
StatePublished - Oct 30 2009

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Keywords

  • RNA folding
  • SAXS
  • counterion
  • ribozyme
  • singular value decomposition

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Moghaddam, S., Caliskan, G., Chauhan, S., Hyeon, C., Briber, R. M., Thirumalai, D., & Woodson, S. A. (2009). Metal Ion Dependence of Cooperative Collapse Transitions in RNA. Journal of molecular biology, 393(3), 753-764. https://doi.org/10.1016/j.jmb.2009.08.044