Metal ion chaperone function of the soluble Cu(I) receptor Atx1

R. A. Pufahl, C. P. Singer, K. L. Peariso, S. J. Lin, P. J. Schmidt, C. J. Fahrni, Valeria L Culotta, J. E. Penner-Hahn, T. V. O'Halloran

Research output: Contribution to journalArticle

Abstract

Reactive and potentially toxic cofactors such as copper ions are imported into eukaryotic cells and incorporated into target proteins by unknown mechanisms. Atx1, a prototypical copper chaperone protein from yeast, has now been shown to act as a soluble cytoplasmic copper(I) receptor that can adopt either a two- or three-coordinate metal center in the active site. Atx1 also associated directly with the Atx1-like cytosolic domains of Ccc2, a vesicular protein defined in genetic studies as a member of the copper- trafficking pathway. The unusual structure and dynamics of Atx1 suggest a copper exchange function for this protein and related domains in the Menkes and Wilson disease proteins.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalScience
Volume278
Issue number5339
DOIs
StatePublished - Oct 31 1997

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Copper
Metals
Ions
Menkes Kinky Hair Syndrome
Fungal Proteins
Poisons
Eukaryotic Cells
Catalytic Domain
Proteins

ASJC Scopus subject areas

  • General

Cite this

Pufahl, R. A., Singer, C. P., Peariso, K. L., Lin, S. J., Schmidt, P. J., Fahrni, C. J., ... O'Halloran, T. V. (1997). Metal ion chaperone function of the soluble Cu(I) receptor Atx1. Science, 278(5339), 853-856. https://doi.org/10.1126/science.278.5339.853

Metal ion chaperone function of the soluble Cu(I) receptor Atx1. / Pufahl, R. A.; Singer, C. P.; Peariso, K. L.; Lin, S. J.; Schmidt, P. J.; Fahrni, C. J.; Culotta, Valeria L; Penner-Hahn, J. E.; O'Halloran, T. V.

In: Science, Vol. 278, No. 5339, 31.10.1997, p. 853-856.

Research output: Contribution to journalArticle

Pufahl, RA, Singer, CP, Peariso, KL, Lin, SJ, Schmidt, PJ, Fahrni, CJ, Culotta, VL, Penner-Hahn, JE & O'Halloran, TV 1997, 'Metal ion chaperone function of the soluble Cu(I) receptor Atx1', Science, vol. 278, no. 5339, pp. 853-856. https://doi.org/10.1126/science.278.5339.853
Pufahl RA, Singer CP, Peariso KL, Lin SJ, Schmidt PJ, Fahrni CJ et al. Metal ion chaperone function of the soluble Cu(I) receptor Atx1. Science. 1997 Oct 31;278(5339):853-856. https://doi.org/10.1126/science.278.5339.853
Pufahl, R. A. ; Singer, C. P. ; Peariso, K. L. ; Lin, S. J. ; Schmidt, P. J. ; Fahrni, C. J. ; Culotta, Valeria L ; Penner-Hahn, J. E. ; O'Halloran, T. V. / Metal ion chaperone function of the soluble Cu(I) receptor Atx1. In: Science. 1997 ; Vol. 278, No. 5339. pp. 853-856.
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