TY - JOUR
T1 - Metabolomics specificity of tuberculosis plasma revealed by 1H NMR spectroscopy
AU - Zhou, Aiping
AU - Ni, Jinjing
AU - Xu, Zhihong
AU - Wang, Ying
AU - Zhang, Haomin
AU - Wu, Wenjuan
AU - Lu, Shuihua
AU - Karakousis, Petros C.
AU - Yao, Yu Feng
N1 - Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (No. 81361120383 , No. 31200109 , No. 81201340 , No. 31070114 ), Shanghai Rising-Star Program, Science and Technology Commission of Shanghai Municipality (No. 12QH1401300 ), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning , and AI106613 and HL106786 (NIH).
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Tuberculosis (TB) is a communicable disease of major global importance and causes metabolic disorder of the patients. In a previous study, we found that the plasma metabolite profile of TB patients differs from that of healthy control subjects based on nuclear magnetic resonance (NMR) spectroscopy. In order to evaluate the TB specificity of the metabolite profile, a total of 110 patients, including 40 with diabetes, 40 with malignancy, and 30 with community-acquired pneumonia (CAP), assessed by NMR spectroscopy, and compared to those of patients with TB. Based on the orthogonal partial least-squares discriminant analysis (OPLS-DA), the metabolic profiles of these diseases were significant different, as compared to the healthy controls and TB patients, respectively. The score plots of the OPLS-DA model demonstrated that TB was easily distinguishable from diabetes, CAP and malignancy. Plasma levels of ketone bodies, lactate, and pyruvate were increased in TB patient compared to healthy control, but lower than CAP and malignancy. We conclude that the metabolic profiles were TB-specific and reflected MTB infection. Our results strongly support the NMR spectroscopy-based metabolomics could contribute to an improved understanding of disease mechanisms and may offer clues to new TB clinic diagnosis and therapies.
AB - Tuberculosis (TB) is a communicable disease of major global importance and causes metabolic disorder of the patients. In a previous study, we found that the plasma metabolite profile of TB patients differs from that of healthy control subjects based on nuclear magnetic resonance (NMR) spectroscopy. In order to evaluate the TB specificity of the metabolite profile, a total of 110 patients, including 40 with diabetes, 40 with malignancy, and 30 with community-acquired pneumonia (CAP), assessed by NMR spectroscopy, and compared to those of patients with TB. Based on the orthogonal partial least-squares discriminant analysis (OPLS-DA), the metabolic profiles of these diseases were significant different, as compared to the healthy controls and TB patients, respectively. The score plots of the OPLS-DA model demonstrated that TB was easily distinguishable from diabetes, CAP and malignancy. Plasma levels of ketone bodies, lactate, and pyruvate were increased in TB patient compared to healthy control, but lower than CAP and malignancy. We conclude that the metabolic profiles were TB-specific and reflected MTB infection. Our results strongly support the NMR spectroscopy-based metabolomics could contribute to an improved understanding of disease mechanisms and may offer clues to new TB clinic diagnosis and therapies.
KW - Community-acquired pneumonia
KW - Diabetes
KW - Malignancy
KW - Metabolite
KW - Plasma
KW - Tuberculosis
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U2 - 10.1016/j.tube.2015.02.038
DO - 10.1016/j.tube.2015.02.038
M3 - Article
C2 - 25736521
AN - SCOPUS:84929262099
VL - 95
SP - 294
EP - 302
JO - Bulletin of the International Union Against Tuberculosis and Lung Disease
JF - Bulletin of the International Union Against Tuberculosis and Lung Disease
SN - 1472-9792
IS - 3
ER -