TY - JOUR
T1 - Metabolomic profiling of urine
T2 - Response to a randomised, controlled feeding study of select fruits and vegetables, and application to an observational study
AU - May, Damon H.
AU - Navarro, Sandi L.
AU - Ruczinski, Ingo
AU - Hogan, Jason
AU - Ogata, Yuko
AU - Schwarz, Yvonne
AU - Levy, Lisa
AU - Holzman, Ted
AU - McIntosh, Martin W.
AU - Lampe, Johanna W.
PY - 2013/11/28
Y1 - 2013/11/28
N2 - Metabolomic profiles were used to characterise the effects of consuming a high-phytochemical diet compared with a diet devoid of fruits and vegetables (F&V) in a randomised trial and cross-sectional study. In the trial, 8Â h fasting urine from healthy men (n 5) and women (n 5) was collected after a 2-week randomised, controlled trial of two diet periods: a diet rich in cruciferous vegetables, citrus and soya (F&V), and a fruit-and vegetable-free (basal) diet. Among the ions found to differentiate the diets, 176 were putatively annotated with compound identifications, with forty-six supported by MS/MS fragment evidence. Metabolites more abundant in the F&V diet included markers of the dietary intervention (e.g. crucifers, citrus and soya), fatty acids and niacin metabolites. Ions more abundant in the basal diet included riboflavin, several acylcarnitines and amino acid metabolites. In the cross-sectional study, we compared the participants based on the tertiles of crucifers, citrus and soya from 3Â d food records (n 36) and FFQ (n 57); intake was separately divided into the tertiles of total fruit and vegetable intake for FFQ. As a group, ions individually differential between the experimental diets differentiated the observational study participants. However, only four ions were significant individually, differentiating the third v. first tertile of crucifer, citrus and soya intake based on 3Â d food records. One of these ions was putatively annotated: proline betaine, a marker of citrus consumption. There were no ions significantly distinguishing tertiles by FFQ. The metabolomic assessment of controlled dietary interventions provides a more accurate and stronger characterisation of the diet than observational data.
AB - Metabolomic profiles were used to characterise the effects of consuming a high-phytochemical diet compared with a diet devoid of fruits and vegetables (F&V) in a randomised trial and cross-sectional study. In the trial, 8Â h fasting urine from healthy men (n 5) and women (n 5) was collected after a 2-week randomised, controlled trial of two diet periods: a diet rich in cruciferous vegetables, citrus and soya (F&V), and a fruit-and vegetable-free (basal) diet. Among the ions found to differentiate the diets, 176 were putatively annotated with compound identifications, with forty-six supported by MS/MS fragment evidence. Metabolites more abundant in the F&V diet included markers of the dietary intervention (e.g. crucifers, citrus and soya), fatty acids and niacin metabolites. Ions more abundant in the basal diet included riboflavin, several acylcarnitines and amino acid metabolites. In the cross-sectional study, we compared the participants based on the tertiles of crucifers, citrus and soya from 3Â d food records (n 36) and FFQ (n 57); intake was separately divided into the tertiles of total fruit and vegetable intake for FFQ. As a group, ions individually differential between the experimental diets differentiated the observational study participants. However, only four ions were significant individually, differentiating the third v. first tertile of crucifer, citrus and soya intake based on 3Â d food records. One of these ions was putatively annotated: proline betaine, a marker of citrus consumption. There were no ions significantly distinguishing tertiles by FFQ. The metabolomic assessment of controlled dietary interventions provides a more accurate and stronger characterisation of the diet than observational data.
KW - Citrus fruit
KW - Cruciferous vegetables
KW - Dietary interventions
KW - FFQ
KW - Mass spectrometry
KW - Metabolomics
KW - Soya
UR - http://www.scopus.com/inward/record.url?scp=84887239094&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887239094&partnerID=8YFLogxK
U2 - 10.1017/S000711451300127X
DO - 10.1017/S000711451300127X
M3 - Article
C2 - 23657156
AN - SCOPUS:84887239094
SN - 0007-1145
VL - 110
SP - 1760
EP - 1770
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 10
ER -