Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study

Santosh Kumar Bharti, Virendra Jaiswal, Ujjala Ghoshal, Uday Chand Ghoshal, Sanjay S. Baijal, Raja Roy, Chunni Lal Khetrapal

Research output: Contribution to journalArticlepeer-review


Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.

Original languageEnglish (US)
Pages (from-to)540-555
Number of pages16
Issue number4
StatePublished - Aug 1 2012
Externally publishedYes


  • Amoebic liver abscess
  • Culture
  • Metabolic profiling
  • NMR spectroscopy
  • PCR
  • Principal Component Analysis
  • Pyogenic liver abscess

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry


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