Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study

Santosh Bharti, Virendra Jaiswal, Ujjala Ghoshal, Uday Chand Ghoshal, Sanjay S. Baijal, Raja Roy, Chunni Lal Khetrapal

Research output: Contribution to journalArticle

Abstract

Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.

Original languageEnglish (US)
Pages (from-to)540-555
Number of pages16
JournalMetabolomics
Volume8
Issue number4
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

Fingerprint

Pyogenic Liver Abscess
Amoebic Liver Abscess
Metabolomics
Liver
Nuclear magnetic resonance
Liver Abscess
formic acid
Polymerase chain reaction
Polymerase Chain Reaction
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
Entamoeba histolytica
Metabolome
Suppuration
Butyrates
Asparagine
Propionates
Succinic Acid
Galactose
Aspartic Acid

Keywords

  • Amoebic liver abscess
  • Culture
  • Metabolic profiling
  • NMR spectroscopy
  • PCR
  • Principal Component Analysis
  • Pyogenic liver abscess

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry

Cite this

Bharti, S., Jaiswal, V., Ghoshal, U., Ghoshal, U. C., Baijal, S. S., Roy, R., & Khetrapal, C. L. (2012). Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study. Metabolomics, 8(4), 540-555. https://doi.org/10.1007/s11306-011-0339-7

Metabolomic profiling of amoebic and pyogenic liver abscesses : An in vitro NMR study. / Bharti, Santosh; Jaiswal, Virendra; Ghoshal, Ujjala; Ghoshal, Uday Chand; Baijal, Sanjay S.; Roy, Raja; Khetrapal, Chunni Lal.

In: Metabolomics, Vol. 8, No. 4, 01.08.2012, p. 540-555.

Research output: Contribution to journalArticle

Bharti, S, Jaiswal, V, Ghoshal, U, Ghoshal, UC, Baijal, SS, Roy, R & Khetrapal, CL 2012, 'Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study', Metabolomics, vol. 8, no. 4, pp. 540-555. https://doi.org/10.1007/s11306-011-0339-7
Bharti, Santosh ; Jaiswal, Virendra ; Ghoshal, Ujjala ; Ghoshal, Uday Chand ; Baijal, Sanjay S. ; Roy, Raja ; Khetrapal, Chunni Lal. / Metabolomic profiling of amoebic and pyogenic liver abscesses : An in vitro NMR study. In: Metabolomics. 2012 ; Vol. 8, No. 4. pp. 540-555.
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