Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study

Santosh Kumar Bharti; Virendra Jaiswal; Ujjala Ghoshal; Uday Chand Ghoshal; Sanjay S. Baijal; Raja Roy; Chunni Lal Khetrapal

doi:10.1007/s11306-011-0339-7

Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study

Santosh Kumar Bharti, Virendra Jaiswal, Ujjala Ghoshal, Uday Chand Ghoshal, Sanjay S. Baijal, Raja Roy, Chunni Lal Khetrapal

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.

Original language	English (US)
Pages (from-to)	540-555
Number of pages	16
Journal	Metabolomics
Volume	8
Issue number	4
DOIs	https://doi.org/10.1007/s11306-011-0339-7
State	Published - Aug 2012
Externally published	Yes

Keywords

Amoebic liver abscess
Culture
Metabolic profiling
NMR spectroscopy
PCR
Principal Component Analysis
Pyogenic liver abscess

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Clinical Biochemistry

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title = "Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study",

abstract = "Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.",

keywords = "Amoebic liver abscess, Culture, Metabolic profiling, NMR spectroscopy, PCR, Principal Component Analysis, Pyogenic liver abscess",

author = "Bharti, {Santosh Kumar} and Virendra Jaiswal and Ujjala Ghoshal and Ghoshal, {Uday Chand} and Baijal, {Sanjay S.} and Raja Roy and Khetrapal, {Chunni Lal}",

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T1 - Metabolomic profiling of amoebic and pyogenic liver abscesses

T2 - An in vitro NMR study

AU - Bharti, Santosh Kumar

AU - Jaiswal, Virendra

AU - Ghoshal, Ujjala

AU - Ghoshal, Uday Chand

AU - Baijal, Sanjay S.

AU - Roy, Raja

AU - Khetrapal, Chunni Lal

PY - 2012/8

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N2 - Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.

AB - Pus samples obtained from 109 patients with liver abscess were examined by NMR spectroscopy. To our knowledge this is the first report on metabolic profiling of liver abscesses. Fifty metabolites were identified by combination of one (1D) and two-dimensional (2D) NMR spectra. Metabolic derangements were evaluated for differentiation between amoebic (ALA) and pyogenic liver abscess (PLA). The NMR results indicate that aspartate, asparagine and galactose, integral components of lipoproteophophoglycans (LPG) of the cell wall of Entamoeba histolytica are metabolic biomarkers of ALA. On the other hand, acetate, propionate, butyrate, succinate and formate, the fermentation products the facultative anaerobes are significantly prevalent in PLA. The NMR based metabolic profile of ALA and PLA are evaluated taking polymerase chain reaction (PCR) and bacterial culture as gold standard method. However, when NMR results were compared with culture and PCR methods, a correct diagnosis of 94. 11% in ALA (n = 85) and 100% in PLA (n = 10) cases were observed. NMR spectroscopy in conjunction with PCR and culture can expedite in differentiating ALA from PLA.

KW - Amoebic liver abscess

KW - Culture

KW - Metabolic profiling

KW - NMR spectroscopy

KW - PCR

KW - Principal Component Analysis

KW - Pyogenic liver abscess

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Metabolomic profiling of amoebic and pyogenic liver abscesses: An in vitro NMR study

Abstract

Keywords

ASJC Scopus subject areas

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