Metabolism of tumor regression fromangiogenesis inhibition: 31P magnetic resonance spectroscopy

F. A. Hoffer, G. A. Taylor, Melissa R Spevak, D. Ingber, T. Fenton

Research output: Contribution to journalArticle

Abstract

31P NMR spectroscopy was used to analyze the in vivo metabolism of reticulum cell sarcoma of mice. The ratio of high- to low-energy phosphates ATP(B) (P(i)) + PME) was measured to reflect the relative metabolic state in the tumor. Of the 34 mice studies, 26 were treated with an antiangiogenesis regimen of heparin and cortisone. Eighty-two-percent of the tumors treated eventually decreased in volume (P <0.01). Volumes and spectroscopic information of 20 tumors were analyzed. Although the average untreated volume was similar to the volume after 3 days of treatment, the average ATP(B)/P(i)) + PME) ratio rose from 0.34 ± 0.10 to 0.47 ± 0.07 (P = 0.02). However, after 6 days of treatment, the volume significantly decreased (P <0.0001) but the ratio did not significantly rise further (P = 0.06). The rise in the high-energy phosphate preceded a significant decrease in volume of the tumors. In addition, the replenishment of the high-energy stores with tumor regression coincided with the histologic findings of a decrease in the number of tumor cells, a decrease of the mitotic index, and a decrease of the number of necrotic cells present with ongoing treatment. Our data suggest that noninvasive methods of assessing early biochemical response of tumor regression may be possible.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalMagnetic Resonance in Medicine
Volume11
Issue number2
StatePublished - 1989
Externally publishedYes

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Magnetic Resonance Spectroscopy
Neoplasms
Cell Count
Adenosine Triphosphate
Phosphates
Mitotic Index
Cortisone
Tumor Burden
Non-Hodgkin's Lymphoma
Heparin

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Metabolism of tumor regression fromangiogenesis inhibition : 31P magnetic resonance spectroscopy. / Hoffer, F. A.; Taylor, G. A.; Spevak, Melissa R; Ingber, D.; Fenton, T.

In: Magnetic Resonance in Medicine, Vol. 11, No. 2, 1989, p. 202-208.

Research output: Contribution to journalArticle

Hoffer, F. A. ; Taylor, G. A. ; Spevak, Melissa R ; Ingber, D. ; Fenton, T. / Metabolism of tumor regression fromangiogenesis inhibition : 31P magnetic resonance spectroscopy. In: Magnetic Resonance in Medicine. 1989 ; Vol. 11, No. 2. pp. 202-208.
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