TY - JOUR
T1 - Metabolism of sulfatides. I. The effect of galactocerebrosides on the synthesis of sulfatides
AU - McKhann, G. M.
AU - Levy, R.
AU - Ho, W.
N1 - Funding Information:
was solubilised from the lCC,OOO x g sediment by resuspending the pellet in desoxycholate (5 mgm/ml) and sonicating for two minutes in a Ratheon sonicator. The sonicated particles were centrifuged at 100,000 x g for 45 minutes, and the resulting supernate used as Y This work was supported by grants NB-05300-01 and gT1 I&Q of the United States Public Health Service.
PY - 1965/7/12
Y1 - 1965/7/12
N2 - Sulfatides, the 3′-sulfate esters of galactocerebrosides, are components of the mammalian myelin sheath. The synthesis of these lipids can be followed by measuring the incorporation of S35-sulfate into the lipid fraction of brain (Davison and Gregson, 1962). In the rat cerebrum, the period of active synthesis of sulfatides invivo (Davison and Gregson, 1962) and invitro (McKhann, et al, 1965) coincides with the onset of the histological appearance of myelin. Despite the close chemical similarity of galactocerebrosides and sulfatides, their metabolic relationships have not been established. The studies reported in this paper indicate that galactocerebrosides are sulfated by a soluble enzyme obtained from the microsomal fraction of rat brain.
AB - Sulfatides, the 3′-sulfate esters of galactocerebrosides, are components of the mammalian myelin sheath. The synthesis of these lipids can be followed by measuring the incorporation of S35-sulfate into the lipid fraction of brain (Davison and Gregson, 1962). In the rat cerebrum, the period of active synthesis of sulfatides invivo (Davison and Gregson, 1962) and invitro (McKhann, et al, 1965) coincides with the onset of the histological appearance of myelin. Despite the close chemical similarity of galactocerebrosides and sulfatides, their metabolic relationships have not been established. The studies reported in this paper indicate that galactocerebrosides are sulfated by a soluble enzyme obtained from the microsomal fraction of rat brain.
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U2 - 10.1016/0006-291X(65)90331-1
DO - 10.1016/0006-291X(65)90331-1
M3 - Article
C2 - 5850675
AN - SCOPUS:0013846682
SN - 0006-291X
VL - 20
SP - 109
EP - 113
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -