Metabolism of leukotriene B4 in the monkey. Identification of the principal nonvolatile metabolite in the urine

William E. Serafin, John A. Oates, Walter C. Hubbard

Research output: Contribution to journalArticle

Abstract

Five milligrams of [5,6,8,9,11,12,14,15-3H8]-leukotriene B4 (LTB4) (1.68 Ci/mmol) were infused into a monkey over a three hour period. Twenty-five per cent of the infused 3H-activity was recovered in the urine during the twenty hours of collection. Plasma and urinary metabolite volatility studies revealed that in contrast to previously studied eicosanoids, more than 70% per cent of the infused LTB4 3H-label was converted to tritiated water. The major nonvolatile urinary metabolite of LTB4 representing 0.8% of the infused material was identified as 20-OH-LTB4. LTB4 was not excreted in the urine. Other nonvolatile metabolites of LTB4 representing less than 0.4% each of the infused material were isolated from the urine. While there was an adequate quantity of some of these metabolites for partial characterization, there was insufficient material for structural elucidation. Further studies were performed in rabbits in which either LTB4 or the structurally related compound 8,15-dihydroxyeicosatetraenoic acid (8,15-diHETE) were infused intravenously. In these rabbits the metabolism of LTB4 and 8,15-diHETE was similar to that in the monkey with greater than 80% of the infused 3H-activity converted to tritiated water. These studies suggest that leukotriene B4 and structurally related compounds undergo extensive degradation in vivo via the β-oxidation system.

Original languageEnglish (US)
Pages (from-to)899-911
Number of pages13
JournalProstaglandins
Volume27
Issue number6
DOIs
StatePublished - 1984
Externally publishedYes

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Leukotriene B4
Metabolites
Metabolism
Haplorhini
Urine
Rabbits
Volatilization
Acids
Eicosanoids
Water
Labels
Plasmas
Degradation
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Metabolism of leukotriene B4 in the monkey. Identification of the principal nonvolatile metabolite in the urine. / Serafin, William E.; Oates, John A.; Hubbard, Walter C.

In: Prostaglandins, Vol. 27, No. 6, 1984, p. 899-911.

Research output: Contribution to journalArticle

Serafin, William E. ; Oates, John A. ; Hubbard, Walter C. / Metabolism of leukotriene B4 in the monkey. Identification of the principal nonvolatile metabolite in the urine. In: Prostaglandins. 1984 ; Vol. 27, No. 6. pp. 899-911.
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abstract = "Five milligrams of [5,6,8,9,11,12,14,15-3H8]-leukotriene B4 (LTB4) (1.68 Ci/mmol) were infused into a monkey over a three hour period. Twenty-five per cent of the infused 3H-activity was recovered in the urine during the twenty hours of collection. Plasma and urinary metabolite volatility studies revealed that in contrast to previously studied eicosanoids, more than 70{\%} per cent of the infused LTB4 3H-label was converted to tritiated water. The major nonvolatile urinary metabolite of LTB4 representing 0.8{\%} of the infused material was identified as 20-OH-LTB4. LTB4 was not excreted in the urine. Other nonvolatile metabolites of LTB4 representing less than 0.4{\%} each of the infused material were isolated from the urine. While there was an adequate quantity of some of these metabolites for partial characterization, there was insufficient material for structural elucidation. Further studies were performed in rabbits in which either LTB4 or the structurally related compound 8,15-dihydroxyeicosatetraenoic acid (8,15-diHETE) were infused intravenously. In these rabbits the metabolism of LTB4 and 8,15-diHETE was similar to that in the monkey with greater than 80{\%} of the infused 3H-activity converted to tritiated water. These studies suggest that leukotriene B4 and structurally related compounds undergo extensive degradation in vivo via the β-oxidation system.",
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