Metabolism links bacterial biofilms and colon carcinogenesis

Caroline H. Johnson, Christine M. Dejea, David Edler, Linh T. Hoang, Antonio F. Santidrian, Brunhilde H. Felding, Julijana Ivanisevic, Kevin Cho, Elizabeth C. Wick, Elizabeth M. Hechenbleikner, Winnie Uritboonthai, Laura Goetz, Robert A Casero, Andrew Mark Pardoll, James R. White, Gary J. Patti, Cynthia Louise Sears, Gary Siuzdak

Research output: Contribution to journalArticle

Abstract

Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N1, N12-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N1, N12-diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.

Original languageEnglish (US)
Pages (from-to)891-897
Number of pages7
JournalCell Metabolism
Volume21
Issue number6
DOIs
StatePublished - Jun 2 2015

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Biofilms
Colon
Carcinogenesis
Colonic Neoplasms
Neoplasms
Polyamines
Bacterial Structures
Metabolomics
Metabolome
Up-Regulation
Cell Proliferation
Anti-Bacterial Agents
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Johnson, C. H., Dejea, C. M., Edler, D., Hoang, L. T., Santidrian, A. F., Felding, B. H., ... Siuzdak, G. (2015). Metabolism links bacterial biofilms and colon carcinogenesis. Cell Metabolism, 21(6), 891-897. https://doi.org/10.1016/j.cmet.2015.04.011

Metabolism links bacterial biofilms and colon carcinogenesis. / Johnson, Caroline H.; Dejea, Christine M.; Edler, David; Hoang, Linh T.; Santidrian, Antonio F.; Felding, Brunhilde H.; Ivanisevic, Julijana; Cho, Kevin; Wick, Elizabeth C.; Hechenbleikner, Elizabeth M.; Uritboonthai, Winnie; Goetz, Laura; Casero, Robert A; Pardoll, Andrew Mark; White, James R.; Patti, Gary J.; Sears, Cynthia Louise; Siuzdak, Gary.

In: Cell Metabolism, Vol. 21, No. 6, 02.06.2015, p. 891-897.

Research output: Contribution to journalArticle

Johnson, CH, Dejea, CM, Edler, D, Hoang, LT, Santidrian, AF, Felding, BH, Ivanisevic, J, Cho, K, Wick, EC, Hechenbleikner, EM, Uritboonthai, W, Goetz, L, Casero, RA, Pardoll, AM, White, JR, Patti, GJ, Sears, CL & Siuzdak, G 2015, 'Metabolism links bacterial biofilms and colon carcinogenesis', Cell Metabolism, vol. 21, no. 6, pp. 891-897. https://doi.org/10.1016/j.cmet.2015.04.011
Johnson CH, Dejea CM, Edler D, Hoang LT, Santidrian AF, Felding BH et al. Metabolism links bacterial biofilms and colon carcinogenesis. Cell Metabolism. 2015 Jun 2;21(6):891-897. https://doi.org/10.1016/j.cmet.2015.04.011
Johnson, Caroline H. ; Dejea, Christine M. ; Edler, David ; Hoang, Linh T. ; Santidrian, Antonio F. ; Felding, Brunhilde H. ; Ivanisevic, Julijana ; Cho, Kevin ; Wick, Elizabeth C. ; Hechenbleikner, Elizabeth M. ; Uritboonthai, Winnie ; Goetz, Laura ; Casero, Robert A ; Pardoll, Andrew Mark ; White, James R. ; Patti, Gary J. ; Sears, Cynthia Louise ; Siuzdak, Gary. / Metabolism links bacterial biofilms and colon carcinogenesis. In: Cell Metabolism. 2015 ; Vol. 21, No. 6. pp. 891-897.
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