TY - JOUR
T1 - Metabolism links bacterial biofilms and colon carcinogenesis
AU - Johnson, Caroline H.
AU - Dejea, Christine M.
AU - Edler, David
AU - Hoang, Linh T.
AU - Santidrian, Antonio F.
AU - Felding, Brunhilde H.
AU - Ivanisevic, Julijana
AU - Cho, Kevin
AU - Wick, Elizabeth C.
AU - Hechenbleikner, Elizabeth M.
AU - Uritboonthai, Winnie
AU - Goetz, Laura
AU - Casero, Robert A.
AU - Pardoll, Drew M.
AU - White, James R.
AU - Patti, Gary J.
AU - Sears, Cynthia L.
AU - Siuzdak, Gary
N1 - Funding Information:
We thank Katharine Romans, Bert Vogelstein, and Kenneth W. Kinzler for providing samples for these studies and Ruchi Badani and Annemarie Boleij for experimental assistance. We would also like to thank Samejima Keijiro from Tokyo Metropolitan Institute of Medical Science for providing [U- 14 N]- and [U- 15 N]-N 1 , N 12 -diacetylspermine.This work was supported by the California Institute of Regenerative Medicine no. TR1-01219; the US National Institutes of Health nos. R01 CA170737, R24 EY017540, P30 MH062261, RC1 HL101034, P01 DA026146, R01 CA151393, R21 CA170492, K087856, P30 DK089502, P30 CA006973, R01 CA051085, R01 CA098454, and T32AI007417; 300-2344 (Alexander and Margaret Stewart Trust, JHU School of Medicine); and U.S. Dept. Energy nos. FG0207ER64325 and DE-AC0205CH11231.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/6/2
Y1 - 2015/6/2
N2 - Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N1, N12-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N1, N12-diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.
AB - Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N1, N12-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N1, N12-diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.
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U2 - 10.1016/j.cmet.2015.04.011
DO - 10.1016/j.cmet.2015.04.011
M3 - Article
C2 - 25959674
AN - SCOPUS:84930575721
SN - 1550-4131
VL - 21
SP - 891
EP - 897
JO - Cell Metabolism
JF - Cell Metabolism
IS - 6
ER -