Metabolically Active Three-Dimensional Brown Adipose Tissue Engineered from White Adipose-Derived Stem Cells

Jessica P. Yang, Amy E. Anderson, Annemarie McCartney, Xavier Ory, Garret Ma, Elisa Pappalardo, Joel Bader, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Brown adipose tissue (BAT) has a unique capacity to expend calories by decoupling energy expenditure from ATP production, therefore BAT could realize therapeutic potential to treat metabolic diseases such as obesity and type 2 diabetes. Recent studies have investigated markers and function of native BAT, however, successful therapies will rely on methods that supplement the small existing pool of brown adipocytes in adult humans. In this study, we engineered BAT from both human and rat adipose precursors and determined whether these ex vivo constructs could mimic in vivo tissue form and metabolic function. Adipose-derived stem cells (ASCs) were isolated from several sources, human white adipose tissue (WAT), rat WAT, and rat BAT, then differentiated toward both white and brown adipogenic lineages in two-dimensional and three-dimensional (3D) culture conditions. ASCs derived from WAT were successfully differentiated in 3D poly(ethylene glycol) hydrogels into mature adipocytes with BAT phenotype and function, including high uncoupling protein 1 (UCP1) mRNA and protein expression and increased metabolic activity (basal oxygen consumption, proton leak, and maximum respiration). By utilizing this "browning" process, the abundant and accessible WAT stem cell population can be engineered into 3D tissue constructs with the metabolic capacity of native BAT, ultimately for therapeutic intervention in vivo and as a tool for studying BAT and its metabolic properties.

Original languageEnglish (US)
Pages (from-to)253-262
Number of pages10
JournalTissue Engineering - Part A
Volume23
Issue number7-8
DOIs
StatePublished - Apr 1 2017

Fingerprint

Brown Adipose Tissue
Stem cells
Stem Cells
Tissue
White Adipose Tissue
Rats
Brown Adipocytes
Hydrogels
Ethylene Glycol
Metabolic Diseases
Adipocytes
Oxygen Consumption
Type 2 Diabetes Mellitus
Energy Metabolism
Protons
Respiration
Therapeutics
Obesity
Adenosine Triphosphate
Proteins

Keywords

  • 3D hydrogels
  • adipogenesis
  • adipose-derived stem cells
  • brown adipose tissue
  • metabolic profiling
  • oxygen consumption rate

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Metabolically Active Three-Dimensional Brown Adipose Tissue Engineered from White Adipose-Derived Stem Cells. / Yang, Jessica P.; Anderson, Amy E.; McCartney, Annemarie; Ory, Xavier; Ma, Garret; Pappalardo, Elisa; Bader, Joel; Elisseeff, Jennifer Hartt.

In: Tissue Engineering - Part A, Vol. 23, No. 7-8, 01.04.2017, p. 253-262.

Research output: Contribution to journalArticle

Yang, Jessica P. ; Anderson, Amy E. ; McCartney, Annemarie ; Ory, Xavier ; Ma, Garret ; Pappalardo, Elisa ; Bader, Joel ; Elisseeff, Jennifer Hartt. / Metabolically Active Three-Dimensional Brown Adipose Tissue Engineered from White Adipose-Derived Stem Cells. In: Tissue Engineering - Part A. 2017 ; Vol. 23, No. 7-8. pp. 253-262.
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