Metabolic studies in isolated rat liver cells: I. Lipid synthesis

David M. Capuzzi, Simeon Margolis

Research output: Contribution to journalArticle

Abstract

Rat liver cells, isolated by chelate perfusion and extrusion through a tissue press, incorporated labeled acetate into cellular lipids and into lipids released into the suspending medium. Optimal rates of incorporation required supplementation of tris-KCl medium with Mg++, Mn++, succinate, citrate, nicotinamide, Coenzyme A, NADP and glucose-6-phosphate. The rate of acetate incorporation was markedly altered by changes in incubation media; tris-KCl was the most effective buffer. All the major classes of cellular lipids were labeled. ATP, BSA, inorganic phosphate, Ca++, 2,4-dinitrophenol and sodium clofibrate were potent inhibitors of acetate incorporation. When added to the incubation mixture, several hormones altered the rate of acetate incorporation into lipids.

Original languageEnglish (US)
Pages (from-to)601-608
Number of pages8
JournalLipids
Volume6
Issue number8
DOIs
StatePublished - Aug 1 1971

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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