Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2

Leta K. Nutt, Seth S. Margolis, Mette Jensen, Catherine E. Herman, William G. Dunphy, Jeffrey C. Rathmell, Sally Kornbluth

Research output: Contribution to journalArticlepeer-review

196 Scopus citations


Vertebrate female reproduction is limited by the oocyte stockpiles acquired during embryonic development. These are gradually depleted over the organism's lifetime through the process of apoptosis. The timer that triggers this cell death is yet to be identified. We used the Xenopus egg/oocyte system to examine the hypothesis that nutrient stores can regulate oocyte viability. We show that pentose-phosphate-pathway generation of NADPH is critical for oocyte survival and that the target of this regulation is caspase-2, previously shown to be required for oocyte death in mice. Pentose-phosphate-pathway-mediated inhibition of cell death was due to the inhibitory phosphorylation of caspase-2 by calcium/calmodulin-dependent protein kinase II (CaMKII). These data suggest that exhaustion of oocyte nutrients, resulting in an inability to generate NADPH, may contribute to ooctye apoptosis. These data also provide unexpected links between oocyte metabolism, CaMKII, and caspase-2.

Original languageEnglish (US)
Pages (from-to)89-103
Number of pages15
Issue number1
StatePublished - Oct 7 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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