TY - JOUR
T1 - Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2
AU - Nutt, Leta K.
AU - Margolis, Seth S.
AU - Jensen, Mette
AU - Herman, Catherine E.
AU - Dunphy, William G.
AU - Rathmell, Jeffrey C.
AU - Kornbluth, Sally
N1 - Funding Information:
We thank A.R. Means, C. Newgard, J. Chandra, F. Chow, and T. Ribar for helpful discussions. We thank D. Ribar for excellent technical assistance, D. Vandré and D. Weitzel for UCN-01, and V. Dixit for RAIDD. This work was supported by NIH RO1s GM 56518 and CA 102727 to S.K. L.K.N. was supported by an individual NRSA. J.C.R. is the recipient of a Howard Temin Career Development award, a Sidney Kimmel Foundation Scholar award, and a V Foundation Scholarship. S.S.M. is a recipient of the George H. Hitchings Fund for Health Research and Science Education of the Triangle Community Foundation.
PY - 2005/10/7
Y1 - 2005/10/7
N2 - Vertebrate female reproduction is limited by the oocyte stockpiles acquired during embryonic development. These are gradually depleted over the organism's lifetime through the process of apoptosis. The timer that triggers this cell death is yet to be identified. We used the Xenopus egg/oocyte system to examine the hypothesis that nutrient stores can regulate oocyte viability. We show that pentose-phosphate-pathway generation of NADPH is critical for oocyte survival and that the target of this regulation is caspase-2, previously shown to be required for oocyte death in mice. Pentose-phosphate-pathway-mediated inhibition of cell death was due to the inhibitory phosphorylation of caspase-2 by calcium/calmodulin-dependent protein kinase II (CaMKII). These data suggest that exhaustion of oocyte nutrients, resulting in an inability to generate NADPH, may contribute to ooctye apoptosis. These data also provide unexpected links between oocyte metabolism, CaMKII, and caspase-2.
AB - Vertebrate female reproduction is limited by the oocyte stockpiles acquired during embryonic development. These are gradually depleted over the organism's lifetime through the process of apoptosis. The timer that triggers this cell death is yet to be identified. We used the Xenopus egg/oocyte system to examine the hypothesis that nutrient stores can regulate oocyte viability. We show that pentose-phosphate-pathway generation of NADPH is critical for oocyte survival and that the target of this regulation is caspase-2, previously shown to be required for oocyte death in mice. Pentose-phosphate-pathway-mediated inhibition of cell death was due to the inhibitory phosphorylation of caspase-2 by calcium/calmodulin-dependent protein kinase II (CaMKII). These data suggest that exhaustion of oocyte nutrients, resulting in an inability to generate NADPH, may contribute to ooctye apoptosis. These data also provide unexpected links between oocyte metabolism, CaMKII, and caspase-2.
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U2 - 10.1016/j.cell.2005.07.032
DO - 10.1016/j.cell.2005.07.032
M3 - Article
C2 - 16213215
AN - SCOPUS:26244453715
VL - 123
SP - 89
EP - 103
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -