Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source

Prabha Dwivedi; Geoffery Puzon; Maggie Tam; Denis Langlais; Shelley Jackson; Kimberly Kaplan; William F. Siems; Albert J. Schultz; Luying Xun; Amina Woods; Herbert H. Hill

doi:10.1002/jms.1850

Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source

Prabha Dwivedi, Geoffery Puzon, Maggie Tam, Denis Langlais, Shelley Jackson, Kimberly Kaplan, William F. Siems, Albert J. Schultz, Luying Xun, Amina Woods, Herbert H. Hill

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, MS alone has difficulty measuring isobars and chiral, conformational and structural isomers. When a matrix-assisted laser desorption ionization (MALDI) source is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (

Original language	English (US)
Pages (from-to)	1383-1393
Number of pages	11
Journal	Journal of Mass Spectrometry
Volume	45
Issue number	12
DOIs	https://doi.org/10.1002/jms.1850
State	Published - Dec 2010
Externally published	Yes

Keywords

Escherichia coli
ESI
ion mobility-mass spectrometry
MALDI
metabolomics
peak capacity

ASJC Scopus subject areas

Spectroscopy

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10.1002/jms.1850

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abstract = "Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, MS alone has difficulty measuring isobars and chiral, conformational and structural isomers. When a matrix-assisted laser desorption ionization (MALDI) source is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (",

keywords = "Escherichia coli, ESI, ion mobility-mass spectrometry, MALDI, metabolomics, peak capacity",

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AU - Dwivedi, Prabha

AU - Puzon, Geoffery

AU - Tam, Maggie

AU - Langlais, Denis

AU - Jackson, Shelley

AU - Kaplan, Kimberly

AU - Siems, William F.

AU - Schultz, Albert J.

AU - Xun, Luying

AU - Woods, Amina

AU - Hill, Herbert H.

PY - 2010/12

Y1 - 2010/12

N2 - Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, MS alone has difficulty measuring isobars and chiral, conformational and structural isomers. When a matrix-assisted laser desorption ionization (MALDI) source is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (

AB - Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, MS alone has difficulty measuring isobars and chiral, conformational and structural isomers. When a matrix-assisted laser desorption ionization (MALDI) source is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (

KW - Escherichia coli

KW - ESI

KW - ion mobility-mass spectrometry

KW - MALDI

KW - metabolomics

KW - peak capacity

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Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source

Abstract

Keywords

ASJC Scopus subject areas

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