Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source

Prabha Dwivedi, Geoffery Puzon, Maggie Tam, Denis Langlais, Shelley Jackson, Kimberly Kaplan, William F. Siems, Albert J. Schultz, Luying Xun, Amina Woods, Herbert H. Hill

Research output: Contribution to journalArticlepeer-review

Abstract

Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, MS alone has difficulty measuring isobars and chiral, conformational and structural isomers. When a matrix-assisted laser desorption ionization (MALDI) source is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (

Original languageEnglish (US)
Pages (from-to)1383-1393
Number of pages11
JournalJournal of Mass Spectrometry
Volume45
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Keywords

  • Escherichia coli
  • ESI
  • ion mobility-mass spectrometry
  • MALDI
  • metabolomics
  • peak capacity

ASJC Scopus subject areas

  • Spectroscopy

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