TY - JOUR
T1 - Metabolic profiling of Commiphora wightii (guggul) reveals a potential source for pharmaceuticals and nutraceuticals
AU - Bhatia, Anil
AU - Bharti, Santosh K.
AU - Tripathi, Tusha
AU - Mishra, Anuradha
AU - Sidhu, Om P.
AU - Roy, Raja
AU - Nautiyal, Chandra Shekhar
N1 - Funding Information:
The financial support from the Department of Biotechnology ( DBT ), New Delhi, India is gratefully acknowledged. The authors are thankful to Prof. Ganesh Pandey, Director CBMR, (SGPGIMS campus), Lucknow, for providing the needed facilities.
Publisher Copyright:
© 2014 Elsevier Ltd. All right reserved.
PY - 2015/2
Y1 - 2015/2
N2 - Guggul gum resin from Commiphora wightii (syn. Commiphora mukul) has been used for centuries in Ayurveda to treat a variety of ailments. The NMR and GC-MS based non-targeted metabolite profiling identified 118 chemically diverse metabolites including amino acids, fatty acids, organic acids, phenolic acids, pregnane-derivatives, steroids, sterols, sugars, sugar alcohol, terpenoids, and tocopherol from aqueous and non-aqueous extracts of leaves, stem, roots, latex and fruits of C. wightii. Out of 118, 51 structurally diverse aqueous metabolites were characterized by NMR spectroscopy. For the first time quinic acid and myo-inositol were identified as the major metabolites in C. wightii. Very high concentration of quinic acid was found in fruits (553.5 ± 39.38 mg g-1 dry wt.) and leaves (212.9 ± 10.37 mg g-1 dry wt.). Similarly, high concentration of myo-inositol (168.8 ± 13.84 mg g-1 dry wt.) was observed from fruits. The other metabolites of cosmeceutical, medicinal, nutraceutical and industrial significance such as α-tocopherol, n-methylpyrrolidone (NMP), trans-farnesol, prostaglandin F2, protocatechuic, gallic and cinnamic acids were identified from non-aqueous extracts using GC-MS. These important metabolites have thus far not been reported from this plant. Isolation of a fungal endophyte, (Nigrospora sps.) from this plant is the first report. The fungal endophyte produced a substantial quantity of bostrycin and deoxybostrycin known for their antitumor properties. Very high concentrations of quinic acid and myo-inositol in leaves and fruits; a substantial quantity of α-tocopherol and NMP in leaves, trans-farnesol in fruits, bostrycin and deoxybostrycin from its endophyte makes the taxa distinct, since these metabolites with medicinal properties find immense applications as dietary supplements and nutraceuticals.
AB - Guggul gum resin from Commiphora wightii (syn. Commiphora mukul) has been used for centuries in Ayurveda to treat a variety of ailments. The NMR and GC-MS based non-targeted metabolite profiling identified 118 chemically diverse metabolites including amino acids, fatty acids, organic acids, phenolic acids, pregnane-derivatives, steroids, sterols, sugars, sugar alcohol, terpenoids, and tocopherol from aqueous and non-aqueous extracts of leaves, stem, roots, latex and fruits of C. wightii. Out of 118, 51 structurally diverse aqueous metabolites were characterized by NMR spectroscopy. For the first time quinic acid and myo-inositol were identified as the major metabolites in C. wightii. Very high concentration of quinic acid was found in fruits (553.5 ± 39.38 mg g-1 dry wt.) and leaves (212.9 ± 10.37 mg g-1 dry wt.). Similarly, high concentration of myo-inositol (168.8 ± 13.84 mg g-1 dry wt.) was observed from fruits. The other metabolites of cosmeceutical, medicinal, nutraceutical and industrial significance such as α-tocopherol, n-methylpyrrolidone (NMP), trans-farnesol, prostaglandin F2, protocatechuic, gallic and cinnamic acids were identified from non-aqueous extracts using GC-MS. These important metabolites have thus far not been reported from this plant. Isolation of a fungal endophyte, (Nigrospora sps.) from this plant is the first report. The fungal endophyte produced a substantial quantity of bostrycin and deoxybostrycin known for their antitumor properties. Very high concentrations of quinic acid and myo-inositol in leaves and fruits; a substantial quantity of α-tocopherol and NMP in leaves, trans-farnesol in fruits, bostrycin and deoxybostrycin from its endophyte makes the taxa distinct, since these metabolites with medicinal properties find immense applications as dietary supplements and nutraceuticals.
KW - Commiphora wightii
KW - GC-MS
KW - Metabolite profiling
KW - NMR spectroscopy
KW - PCA
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U2 - 10.1016/j.phytochem.2014.12.016
DO - 10.1016/j.phytochem.2014.12.016
M3 - Article
C2 - 25561401
AN - SCOPUS:84921532322
SN - 0031-9422
VL - 110
SP - 29
EP - 36
JO - Phytochemistry
JF - Phytochemistry
ER -