Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications

Christopher T. Saeui, Esteban Urias, Lingshu Liu, Mohit P. Mathew, Kevin J. Yarema

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolic glycoengineering is a specialization of metabolic engineering that focuses on using small molecule metabolites to manipulate biosynthetic pathways responsible for oligosaccharide and glycoconjugate production. As outlined in this article, this technique has blossomed in mammalian systems over the past three decades but has made only modest progress in prokaryotes. Nevertheless, a sufficient foundation now exists to support several important applications of metabolic glycoengineering in bacteria based on methods to preferentially direct metabolic intermediates into pathways involved in lipopolysaccharide, peptidoglycan, teichoic acid, or capsule polysaccharide production. An overview of current applications and future prospects for this technology are provided in this report.

Original languageEnglish (US)
Pages (from-to)425-441
Number of pages17
JournalGlycoconjugate Journal
Volume32
Issue number7
DOIs
StatePublished - Oct 1 2015

Keywords

  • Carbohydrate engineering
  • Lipopolysaccharides
  • Metabolic oligosaccharide engineering
  • Nucleotide sugar production
  • Oligosaccharide synthesis
  • Petidoglycans
  • Recombinant glycoprotein production

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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