Metabolic clearance and production rates of prolactin in man

David S Cooper, E. C. Ridgway, B. Kliman, R. N. Kjellberg, F. Maloof

Research output: Contribution to journalArticle

Abstract

Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m 2 in women and 44±3 ml/min per m 2 in men, and was significantly correlated with body mass (r=0.84, P2, P2, P=NS), and was significantly correlated with serum thyroxine (r=0.46, P2, P2, respectively (P=NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 μg/d per m 2, P2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 μg/d per m 2). Dopamine infusion decreased PRL PR from 270 to 66 μg/d per m 2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism. To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially converted to two larger forms (peaks I and II) in vivo. Peak I (Kav=0) was 30-40% immunoprecipitable, although peak II (Kav=0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in ≥90% conversion to peak III and restoration of full immunoactivity. Thus, peak I is a noncovalently linked aggregate that is partially immunoactive, and therefore able to alter MCR determinations. These studies demonstrate the impact of hormone heterogeneity on MCR estimations and suggest that gel filtration of immunoprecipitable material be an integral part of future MCR measurements.

Original languageEnglish (US)
Pages (from-to)1669-1680
Number of pages12
JournalJournal of Clinical Investigation
Volume64
Issue number6
StatePublished - 1979
Externally publishedYes

Fingerprint

Metabolic Clearance Rate
Prolactin
Hyperprolactinemia
Hyperthyroidism
Gel Chromatography
Dopamine
Hypothyroidism
Serum
Thyroxine
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Hormones

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cooper, D. S., Ridgway, E. C., Kliman, B., Kjellberg, R. N., & Maloof, F. (1979). Metabolic clearance and production rates of prolactin in man. Journal of Clinical Investigation, 64(6), 1669-1680.

Metabolic clearance and production rates of prolactin in man. / Cooper, David S; Ridgway, E. C.; Kliman, B.; Kjellberg, R. N.; Maloof, F.

In: Journal of Clinical Investigation, Vol. 64, No. 6, 1979, p. 1669-1680.

Research output: Contribution to journalArticle

Cooper, DS, Ridgway, EC, Kliman, B, Kjellberg, RN & Maloof, F 1979, 'Metabolic clearance and production rates of prolactin in man', Journal of Clinical Investigation, vol. 64, no. 6, pp. 1669-1680.
Cooper DS, Ridgway EC, Kliman B, Kjellberg RN, Maloof F. Metabolic clearance and production rates of prolactin in man. Journal of Clinical Investigation. 1979;64(6):1669-1680.
Cooper, David S ; Ridgway, E. C. ; Kliman, B. ; Kjellberg, R. N. ; Maloof, F. / Metabolic clearance and production rates of prolactin in man. In: Journal of Clinical Investigation. 1979 ; Vol. 64, No. 6. pp. 1669-1680.
@article{98b6f586b49148e5bf3933f3d4f291a7,
title = "Metabolic clearance and production rates of prolactin in man",
abstract = "Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m 2 in women and 44±3 ml/min per m 2 in men, and was significantly correlated with body mass (r=0.84, P2, P2, P=NS), and was significantly correlated with serum thyroxine (r=0.46, P2, P2, respectively (P=NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 μg/d per m 2, P2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 μg/d per m 2). Dopamine infusion decreased PRL PR from 270 to 66 μg/d per m 2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism. To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially converted to two larger forms (peaks I and II) in vivo. Peak I (Kav=0) was 30-40{\%} immunoprecipitable, although peak II (Kav=0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in ≥90{\%} conversion to peak III and restoration of full immunoactivity. Thus, peak I is a noncovalently linked aggregate that is partially immunoactive, and therefore able to alter MCR determinations. These studies demonstrate the impact of hormone heterogeneity on MCR estimations and suggest that gel filtration of immunoprecipitable material be an integral part of future MCR measurements.",
author = "Cooper, {David S} and Ridgway, {E. C.} and B. Kliman and Kjellberg, {R. N.} and F. Maloof",
year = "1979",
language = "English (US)",
volume = "64",
pages = "1669--1680",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "6",

}

TY - JOUR

T1 - Metabolic clearance and production rates of prolactin in man

AU - Cooper, David S

AU - Ridgway, E. C.

AU - Kliman, B.

AU - Kjellberg, R. N.

AU - Maloof, F.

PY - 1979

Y1 - 1979

N2 - Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m 2 in women and 44±3 ml/min per m 2 in men, and was significantly correlated with body mass (r=0.84, P2, P2, P=NS), and was significantly correlated with serum thyroxine (r=0.46, P2, P2, respectively (P=NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 μg/d per m 2, P2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 μg/d per m 2). Dopamine infusion decreased PRL PR from 270 to 66 μg/d per m 2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism. To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially converted to two larger forms (peaks I and II) in vivo. Peak I (Kav=0) was 30-40% immunoprecipitable, although peak II (Kav=0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in ≥90% conversion to peak III and restoration of full immunoactivity. Thus, peak I is a noncovalently linked aggregate that is partially immunoactive, and therefore able to alter MCR determinations. These studies demonstrate the impact of hormone heterogeneity on MCR estimations and suggest that gel filtration of immunoprecipitable material be an integral part of future MCR measurements.

AB - Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m 2 in women and 44±3 ml/min per m 2 in men, and was significantly correlated with body mass (r=0.84, P2, P2, P=NS), and was significantly correlated with serum thyroxine (r=0.46, P2, P2, respectively (P=NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 μg/d per m 2, P2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 μg/d per m 2). Dopamine infusion decreased PRL PR from 270 to 66 μg/d per m 2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism. To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially converted to two larger forms (peaks I and II) in vivo. Peak I (Kav=0) was 30-40% immunoprecipitable, although peak II (Kav=0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in ≥90% conversion to peak III and restoration of full immunoactivity. Thus, peak I is a noncovalently linked aggregate that is partially immunoactive, and therefore able to alter MCR determinations. These studies demonstrate the impact of hormone heterogeneity on MCR estimations and suggest that gel filtration of immunoprecipitable material be an integral part of future MCR measurements.

UR - http://www.scopus.com/inward/record.url?scp=0018643567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018643567&partnerID=8YFLogxK

M3 - Article

C2 - 583048

AN - SCOPUS:0018643567

VL - 64

SP - 1669

EP - 1680

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 6

ER -